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SERPINE1 elisa kit :: Pig serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1) , member 1 ELISA Kit

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Catalog # MBS703957
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1) , member 1 (SERPINE1), ELISA Kit

Popular Item
Full Product Name

Pig plasminogen activator inhibitor 1, PAI-1 ELISA Kit

Product Synonym Names
Pig plasminogen activator inhibitor 1; PAI-1 ELISA Kit; PAI; PAI-1; PAI1; PLANH1; plasminogen activator inhibitor-1; serine (or cysteine) proteinase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 1; serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703957 Testing Data
3D Structure
ModBase 3D Structure for P79335
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig SERPINE1. No significant cross-reactivity or interference between Pig SERPINE1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SERPINE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SERPINE1purchase
MBS703957 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 (SERPINE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINE1. The ELISA analytical biochemical technique of the MBS703957 kit is based on SERPINE1 antibody-SERPINE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SERPINE1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PAI-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAI-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAI-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAI-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SERPINE1 elisa kit
SERPINE1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703957. Click to request current manual
NCBI/Uniprot data below describe general gene information for SERPINE1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
45,450 Da
NCBI Official Full Name
plasminogen activator inhibitor 1
NCBI Official Symbol
SERPINE1  [Similar Products]
NCBI Official Synonym Symbols
PLANH1
  [Similar Products]
NCBI Protein Information
plasminogen activator inhibitor 1; PAI; PAI-1; serpin E1; plasminogen activator inhibitor I; endothelial plasminogen activator inhibitor
UniProt Protein Name
Plasminogen activator inhibitor 1
UniProt Synonym Protein Names
Endothelial plasminogen activator inhibitor; Serpin E1
UniProt Gene Name
SERPINE1  [Similar Products]
UniProt Synonym Gene Names
PAI1; PLANH1; PAI; PAI-1  [Similar Products]
UniProt Entry Name
PAI1_PIG
UniProt Comments for SERPINE1
Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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