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SERPINF1 elisa kit :: Mouse PEDF ELISA Kit

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Catalog # MBS412006
Unit / Price
  1x96 Wells  /  $510 +1 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

PEDF (SERPINF1), ELISA Kit

Popular Item
Also Known As

Mouse PEDF ELISA kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
418
Species Reactivity
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. Product is stable for at least six months from date of shipment.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SERPINF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSERPINF1purchase
MBS412006 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PEDF (SERPINF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINF1. The ELISA analytical biochemical technique of the MBS412006 kit is based on SERPINF1 antibody-SERPINF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SERPINF1 elisa kit
Principle of the Assay||Microwells are coated with a polyclonal antibody specific for fulllength recombinant mouse PEDF antigen. The PEDF antigen present in the specimen is specifically captured onto the immobilized antibody during specimen incubation. The captured antigen is then detected with a high-titer polyclonal anti-PEDF antibody conjugated to biotin. Following subsequent incubation with streptavidin peroxidase, the bound enzyme reacts with a substrate to yield a colored product. The resultant optical density is proportional to the amount of PEDF antigen present in the specimen.

Typical Testing Data/Standard Curve of SERPINF1 elisa kit
SERPINF1 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS412006. Click to request current manual
NCBI/Uniprot data below describe general gene information for SERPINF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
NCBI Official Full Name
pigment epithelium-derived factor
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
NCBI Official Symbol
SERPINF1  [Similar Products]
NCBI Official Synonym Symbols
OI6; OI12; PEDF; EPC-1; PIG35
  [Similar Products]
NCBI Protein Information
pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
NCBI Summary for SERPINF1
The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]
UniProt Comments for SERPINF1
PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; negative regulation of angiogenesis; positive regulation of neurogenesis; ovulation cycle; retina development in camera-type eye; short-term memory; response to arsenic; multicellular organismal development; negative regulation of inflammatory response; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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