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SFRP5 elisa kit :: Human SFRP5 ELISA Kit

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Catalog # MBS8248400
Unit / Price
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  96 Tests  /  $490 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

SFRP5, ELISA Kit

Popular Item
Full Product Name

Human SFRP5 ELISA Kit

Product Synonym Names
FRP1B; SARP3; Secreted frizzled-related protein 5; sFRP-5; Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3; SARP-3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
AF017988 mRNA
3D Structure
ModBase 3D Structure for Q5T4F7
Species Reactivity
Specificity
The Human SFRP5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human SFRP5 proteins within the range of 156 pg/ml - 10000 pg/ml.
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
<15 pg/mL
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SFRP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SFRP5purchase
MBS8248400 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SFRP5, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SFRP5. The ELISA analytical biochemical technique of the MBS8248400 kit is based on SFRP5 antibody-SFRP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SFRP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SFRP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SFRP5 elisa kit
Principle of the Assay: MDS Human SFRP5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SFRP5 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human SFRP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and SFRP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SFRP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SFRP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Typical Testing Data/Standard Curve (for reference only) of SFRP5 elisa kit
SFRP5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS8248400. Click to request current manual
NCBI/Uniprot data below describe general gene information for SFRP5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,563 Da
NCBI Official Full Name
secreted frizzled-related protein 5
NCBI Official Synonym Full Names
secreted frizzled related protein 5
NCBI Official Symbol
SFRP5  [Similar Products]
NCBI Official Synonym Symbols
SARP3
  [Similar Products]
NCBI Protein Information
secreted frizzled-related protein 5
UniProt Protein Name
Secreted frizzled-related protein 5
UniProt Synonym Protein Names
Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3; SARP-3
UniProt Gene Name
SFRP5  [Similar Products]
UniProt Synonym Gene Names
FRP1B; SARP3; sFRP-5; FRP-1b; SARP-3  [Similar Products]
UniProt Entry Name
SFRP5_HUMAN
NCBI Summary for SFRP5
Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq, Jul 2008]
UniProt Comments for SFRP5
SFRP5: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: extracellular space; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt receptor activity; Wnt-protein binding

Biological Process: anatomical structure morphogenesis; apoptosis; establishment and/or maintenance of cell polarity; negative regulation of cell proliferation; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; signal transduction; visual perception
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with SFRP5 elisa kit
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