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Sgk2 elisa kit :: Mouse Serine/threonine-protein kinase Sgk2 ELISA Kit

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Catalog # MBS2886412
Unit / Price
  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Serine/threonine-protein kinase Sgk2, ELISA Kit

Popular Item
Also Known As

Mouse Serine/threonine-protein kinase Sgk2 ELISA Kit

Product Synonym Names
Serine/threonine-protein kinase Sgk2; Serum/glucocorticoid-regulated kinase 2; Sgk2; 2.7.11.1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2886412 Testing Data
Sequence Length
366
3D Structure
ModBase 3D Structure for Q9QZS5
Species Reactivity
Specificity
Recombinant and natural Mouse Serine/threonine-protein kinase Sgk2
Assay Type
Competitive
Detection Range
0.78-50 ng/mL
Sensitivity
0.415 ng/mL
Intra-Assay CV
<=5.4%
Inter-Assay CV
<=7.1%
Recovery
80%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Sgk2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSgk2purchase
MBS2886412 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Serine/threonine-protein kinase Sgk2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Sgk2. The ELISA analytical biochemical technique of the MBS2886412 kit is based on Sgk2 antibody-Sgk2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Sgk2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Sgk2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Sgk2 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Sgk2 elisa kit
Sgk2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2886412. Click to request current manual
NCBI/Uniprot data below describe general gene information for Sgk2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,248 Da
NCBI Official Full Name
serine/threonine-protein kinase Sgk2 isoform b
NCBI Official Synonym Full Names
serum/glucocorticoid regulated kinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
Sgkl; AI098171; AW146006
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NCBI Protein Information
serine/threonine-protein kinase Sgk2
UniProt Protein Name
Serine/threonine-protein kinase Sgk2
UniProt Synonym Protein Names
Serum/glucocorticoid-regulated kinase 2
UniProt Gene Name
UniProt Entry Name
SGK2_MOUSE
UniProt Comments for Sgk2
SGK2: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up- regulates Na(+) channels: SCNN1A/ENAC, K(+) channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Highly expressed in liver, kidney and pancreas, and at lower levels in brain. Two specific sites, one in the kinase domain (Thr-253) and the other in the C-terminal regulatory region (Ser- 416), need to be phsphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.1; AGC group; SGK family

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: ATP binding; calcium channel regulator activity; chloride channel regulator activity; kinase activity; nucleotide binding; potassium channel regulator activity; protein kinase activity; protein serine/threonine kinase activity; sodium channel regulator activity; transferase activity

Biological Process: cellular sodium ion homeostasis; peptidyl-serine phosphorylation; phosphorylation; positive regulation of transporter activity; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell growth; regulation of cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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