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SGLT2 elisa kit :: Human Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit

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Catalog # MBS2023344 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $665 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Sodium/Glucose Cotransporter 2 (SGLT2), ELISA Kit

Also Known As

Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit

Product Synonym Names
SLC5A2; Solute Carrier Family 5 Member 2; Low affinity sodium-glucose cotransporter; Na(+)/glucose cotransporter 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
458
OMIM
182381
3D Structure
ModBase 3D Structure for P31639
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Sodium/Glucose Cotransporter 2 (SGLT2).
No significant cross-reactivity or interference between Sodium/Glucose Cotransporter 2 (SGLT2) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.78-50ng/mL
Sensitivity
< 0.32ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Sodium/Glucose Cotransporter 2 (SGLT2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Sodium/Glucose Cotransporter 2 (SGLT2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SGLT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSGLT2purchase
MBS2023344 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SGLT2. The ELISA analytical biochemical technique of the MBS2023344 kit is based on SGLT2 antibody-SGLT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SGLT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SGLT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SGLT2 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Glucose Cotransporter 2 (SGLT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Glucose Cotransporter 2 (SGLT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Glucose Cotransporter 2 (SGLT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Glucose Cotransporter 2 (SGLT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for SGLT2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of SGLT2 elisa kit
SGLT2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2023344. Click to request current manual
NCBI/Uniprot data below describe general gene information for SGLT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,357 Da
NCBI Official Full Name
sodium/glucose cotransporter 2
NCBI Official Synonym Full Names
solute carrier family 5 (sodium/glucose cotransporter), member 2
NCBI Official Symbol
SLC5A2  [Similar Products]
NCBI Official Synonym Symbols
SGLT2
  [Similar Products]
NCBI Protein Information
sodium/glucose cotransporter 2
UniProt Protein Name
Sodium/glucose cotransporter 2
UniProt Synonym Protein Names
Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2
UniProt Gene Name
SLC5A2  [Similar Products]
UniProt Synonym Gene Names
SGLT2; Na(+)/glucose cotransporter 2  [Similar Products]
UniProt Entry Name
SC5A2_HUMAN
NCBI Summary for SGLT2
This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene. [provided by RefSeq, Feb 2015]
UniProt Comments for SGLT2
SLC5A2: Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1. Defects in SLC5A2 are the cause of renal glucosuria (GLYS1). GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: low-affinity glucose:sodium symporter activity

Biological Process: transport; carbohydrate metabolic process; sodium ion transport; glucose transport; transmembrane transport

Disease: Renal Glucosuria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withSGLT2 elisa kit
 Reference Product  PubMed Publications
 SLC2A2 elisa kit  >2 publications with SGLT2 and SLC2A2
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