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SHBG elisa kit :: Mouse sex hormone-binding globulin ELISA Kit

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Catalog # MBS703219
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $575 +1 FREE 8GB USB
  96-Strip-Wells  /  $835 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

sex hormone-binding globulin (SHBG), ELISA Kit

Also Known As

Mouse sex hormone-binding globulin, SHBG ELISA Kit

Product Synonym Names
Mouse sex hormone-binding globulin; SHBG ELISA Kit; ABP; MGC126834; MGC138391; SBP; TEBG; androgen binding protein; sex steroid-binding protein; testis-specific androgen-binding protein; testosterone-binding beta-globulin; testosterone-estradiol-binding; sex hormone-binding globulin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703219 Testing Data
Sequence Length
403
3D Structure
ModBase 3D Structure for P97497
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse SHBG. No significant cross-reactivity or interference between Mouse SHBG and analogues was observed.
Detection Range
1.7 ng/ml-50 ng/ml
Sensitivity
0.4 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SHBG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSHBGpurchase
MBS703219 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sex hormone-binding globulin (SHBG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SHBG. The ELISA analytical biochemical technique of the MBS703219 kit is based on SHBG antibody-SHBG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SHBG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SHBG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SHBG elisa kit
Introduction: Sex hormone-binding globulin (SHBG) is a glycoprotein that binds to sex hormones, specifically testosterone and estradiol. Other steroid hormones such as progesterone, cortisol, and other corticosteroids are bound by transcortin. SHBG is produced by the liver cells and is released into the bloodstream. Other sites that produce SHBG are the brain, uterus, and placenta. In addition SHBG is produced by the testes; testes-produced SHBG is also called androgen-binding protein. The gene for SHBG is located on chromosome 17. SHBG levels appear to be controlled by a delicate balance of enhancing and inhibiting factors. Its level is decreased by high levels of insulin and insulin-like growth factor 1 (IGF-1). Also, high androgen and transcortin levels decrease SHBG, while high growth hormone, estrogen and thyroxine levels increase it. However, recent evidence suggests that it is the liver's production of fats that reduces SHBG levels, not any direct effect of insulin and specific genetic mechanisms have been found that do this.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHBG antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated monoclonal antibody preparation specific for SHBG and incubated. Then substrate solution A and B are added to each well. Only those wells that contain SHBG, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SHBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of SHBG elisa kit
SHBG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703219. Click to request current manual
NCBI/Uniprot data below describe general gene information for SHBG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,816 Da
NCBI Official Full Name
sex hormone-binding globulin
NCBI Official Synonym Full Names
sex hormone binding globulin
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
sex hormone-binding globulin; SBP; sex steroid-binding protein; testis-specific androgen-binding protein
UniProt Protein Name
Sex hormone-binding globulin
UniProt Synonym Protein Names
Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP
UniProt Gene Name
UniProt Synonym Gene Names
SHBG; SBP; ABP  [Similar Products]
UniProt Entry Name
SHBG_MOUSE
UniProt Comments for SHBG
Function: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with SHBG elisa kit
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