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SHLA recombinant protein :: super leptin antagonist Recombinant Protein

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Catalog # MBS400098
Unit / Price
  0.05 mg  /  $165 +1 FREE 8GB USB
  0.1 mg  /  $215 +1 FREE 8GB USB
  0.5 mg  /  $610 +1 FREE 8GB USB
  1 mg  /  $965 +1 FREE 8GB USB
  5 mg  /  $3,625 +4 FREE 8GB USB
  10 mg  /  $6,205 +7 FREE 8GB USB
SHLA recombinant protein
Product Name

super leptin antagonist (SHLA), Recombinant Protein

Popular Item
Also Known As

Human super leptin antagonist (mutant D23/L39A/D40A/F41A)

Product Synonym Names
Human super-active leptin antagonist (SHLA)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The sequence of the first five N-terminal amino of acids of super human leptin antagonist was determined and was found to be Ala-Val-Pr o-Ile-Gln
Host
E Coli
Purity/Purification
The purity of super human leptin antagonist is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized super human leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.
Protein Content
Protein quantization of super human leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified super human leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.02 ng/ug (0.2 EU/ug) of super human leptin antagonist
Formulation
The recombinant protein was lyophilized from a concentrated (1 mg/ml) solution with 0.003mM NaHCO3.
Biological Activity
super human leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Super human leptin antagonist also inhibits various leptin effects in several in vitro bioassays. The inhibitory activity of super human leptin antagonist was increased 14 to 60 fold as measured by various criteria such as binding properties to human leptin binding domain (hLBD) and in vitro and in vivo bioassays.
Preparation and Storage
Lyophilized super human leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -20 degree C. Upon reconstitution at of super human leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization super human leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Other Notes
Small volumes of SHLA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SHLA recombinant protein
Recombinant super human leptin antagonist is one polypeptide chain containing 146 amino. Super human leptin antagonist (SHLA) was initially mutated, resulting in D23L/L39A/D40A/F41A super human leptin antagonist that was purified by proprietary chromatographic techniques. More details can be found in Shpilman at al., J. Biol. Chem. 286:4439-42 (2011).
Product Categories/Family for SHLA recombinant protein
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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