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Add To Compare List sIGF1 active protein :: Gilthead Seabream Insulin Like Growth Factor-1 Active Protein Catalog # MBS142172 Unit / Price 0.01 mg / $245 +1 FREE 8GB USB 0.05 mg / $320 +1 FREE 8GB USB 1 mg / $2,280 +2 FREE 8GB USB 5x1 mg / $9,925 +10 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Gilthead Seabream Insulin Like Growth Factor-1 (sIGF1), Active Protein Popular Item Full Product Name Recombinant Gilthead Seabream Insulin Like Growth Factor-1 Product Synonym Names IGF1 Gilthead Seabream; Insulin-Like Growth Factor 1 Gilthead Seabream Recombinant; Somatomedin C; IGF-I; IGFI Product Gene Name sIGF1 active protein[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP MBS142172 Technical Datasheet Technical Datasheet PDF Sequence The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ser-Pr o-Glu-Thr- Leu-Cys-Gl y-Ala-Glu Host E Coli Purity/Purification Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. Form/Format The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.Sterile Filtered White lyophilized (freeze-dried) powder. Solubility It is recommended to reconstitute the lyophilized IGF-1 in sterile 0.4% NaHCO3 adjusted, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content Somatomedin C quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.60 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard. Biological Activity Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active. Preparation and Storage Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IGF1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Active Protein Note Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product. Other Notes Small volumes of sIGF1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for sIGF1 active protein Description: Insulin-Like Growth Factor-IGilthead SeabreamRecombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7545.4 Dalton, the predicted pI=7.72.IGF-1 is purified by proprietary chromatographic techniques.Introduction: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003). Product Categories/Family for sIGF1 active protein Cytokines and Growth Factors; Growth Factors; Insulin-Like Growth Factor Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP