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SIK2 elisa kit :: Human Serine/threonine protein kinase SIK2 ELISA Kit

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Catalog # MBS2533324
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Serine/threonine protein kinase SIK2, ELISA Kit

Popular Item
Full Product Name

Human SIK2 (Serine/threonine protein kinase SIK2) ELISA Kit

Product Synonym Names
Salt Inducible Kinase 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
608973
3D Structure
ModBase 3D Structure for Q9H0K1
Species Reactivity
Specificity
This kit recognizes natural and recombinantHumanItgam/CD11b. No significant cross-reactivity or interference between HumanItgam/CD11b and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
Min: 0.375ng/mL; Max: 40ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SIK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SIK2purchase
MBS2533324 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Serine/threonine protein kinase SIK2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SIK2. The ELISA analytical biochemical technique of the MBS2533324 kit is based on SIK2 antibody-SIK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SIK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SIK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SIK2 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanItgam/CD11bconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Itgam/CD11b. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Itgam/CD11band Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Itgam/CD11b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Itgam/CD11b.You can calculate the concentration of Itgam/CD11bin the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of SIK2 elisa kit
SIK2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2533324. Click to request current manual
NCBI/Uniprot data below describe general gene information for SIK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
103,915 Da
NCBI Official Full Name
serine/threonine-protein kinase SIK2
NCBI Official Synonym Full Names
salt inducible kinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
QIK; SIK-2; SNF1LK2; LOH11CR1I
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase SIK2
UniProt Protein Name
Serine/threonine-protein kinase SIK2
UniProt Synonym Protein Names
Qin-induced kinase; Salt-inducible kinase 2; SIK-2; Serine/threonine-protein kinase SNF1-like kinase 2
UniProt Gene Name
UniProt Synonym Gene Names
; SIK-2  [Similar Products]
UniProt Entry Name
SIK2_HUMAN
UniProt Comments for SIK2
QIK: a serine/threonine kinase of the CAMKL family and related to AMPK. Specifically expressed in adipose tissues. Like AMPK, QIK is phosphorylated and activated by LKB1. Part of a molecular complex including TORC2 and calcineurin that regulates the effects of circulating glucose and gut hormones during feeding on TORC2-mediated gene expression. In mice, insulin promotes the phosphorylation and ubiquitin-dependent degradation of TORC2, inhibiting gluconeogenic gene expression during re-feeding. In response to insulin, Akt2 phosphorylates and activates QIK which in turn stimulates the phosphorylation and cytoplasmic translocation of TORC2 where it associates with COP1, which promotes its ubiquitination and proteasomal degradation. Phosphorylates Ser794 of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; CAMKL family; QIK subfamily

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; magnesium ion binding; protein binding; protein serine/threonine kinase activity

Biological Process: insulin receptor signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of insulin receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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