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SIRT1O elisa kit :: Human NAD-dependent deacetylase sirtuin-1 ELISA Kit

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Catalog # MBS288506
Unit / Price
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  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

NAD-dependent deacetylase sirtuin-1 (SIRT1O), ELISA Kit

Popular Item
Full Product Name

Human NAD-dependent deacetylase sirtuin-1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS288506 Testing Data
OMIM
604479
3D Structure
ModBase 3D Structure for Q96EB6
Species Reactivity
Assay Type
Sandwich
Detection Range
78-5000 pg/mL
Sensitivity
32pg/mL
Intra-Assay CV
<=4.3%
Inter-Assay CV
<=7.2%
Recovery
85%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SIRT1O elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SIRT1Opurchase
MBS288506 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NAD-dependent deacetylase sirtuin-1 (SIRT1O) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SIRT1O. The ELISA analytical biochemical technique of the MBS288506 kit is based on SIRT1O antibody-SIRT1O antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SIRT1O antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SIRT1O. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SIRT1O elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of SIRT1O elisa kit
SIRT1O elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS288506. Click to request current manual
NCBI/Uniprot data below describe general gene information for SIRT1O. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,066 Da
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-1 isoform b
NCBI Official Synonym Full Names
sirtuin 1
NCBI Official Symbol
SIRT1  [Similar Products]
NCBI Official Synonym Symbols
SIR2; SIR2L1; SIR2alpha
  [Similar Products]
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-1
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-1
UniProt Synonym Protein Names
Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2
UniProt Gene Name
SIRT1  [Similar Products]
UniProt Synonym Gene Names
SIR2L1; hSIRT1; hSIR2; 75SirT1  [Similar Products]
UniProt Entry Name
SIR1_HUMAN
NCBI Summary for SIRT1O
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
UniProt Comments for SIRT1O
SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-; Nuclear receptor co-regulator; Deacetylase; Apoptosis

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: chromatin silencing complex; cytoplasm; ESC/E(Z) complex; mitochondrion; nuclear chromatin; nuclear envelope; nuclear heterochromatin; nuclear inner membrane; nucleolus; nucleoplasm; nucleus; PML body

Molecular Function: bHLH transcription factor binding; deacetylase activity; enzyme binding; histone binding; histone deacetylase activity; HLH domain binding; identical protein binding; metal ion binding; mitogen-activated protein kinase binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); nuclear hormone receptor binding; p53 binding; protein binding; protein C-terminus binding; protein deacetylase activity; transcription corepressor activity; transcription factor binding

Biological Process: angiogenesis; behavioral response to starvation; cell aging; cell glucose homeostasis; cellular response to starvation; cholesterol homeostasis; chromatin silencing; chromatin silencing at rDNA; circadian regulation of gene expression; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA repair; DNA replication; DNA synthesis during DNA repair; establishment and/or maintenance of chromatin architecture; establishment of chromatin silencing; fatty acid homeostasis; gene expression; histone deacetylation; inhibition of NF-kappaB transcription factor; leptin-mediated signaling pathway; maintenance of chromatin silencing; methylation-dependent chromatin silencing; muscle development; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of fat cell differentiation; negative regulation of gene expression, epigenetic; negative regulation of helicase activity; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of phosphorylation; negative regulation of prostaglandin biosynthetic process; negative regulation of protein kinase B signaling cascade; negative regulation of TOR signaling pathway; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; ovulation from ovarian follicle; peptidyl-lysine acetylation; positive regulation of adaptive immune response; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell proliferation; positive regulation of chromatin silencing; positive regulation of DNA repair; positive regulation of endothelial cell proliferation; positive regulation of histone H3-K9 methylation; positive regulation of insulin receptor signaling pathway; positive regulation of macroautophagy; positive regulation of MHC class II biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid ADP-ribosylation; protein amino acid deacetylation; protein destabilization; protein ubiquitination; pyrimidine dimer repair via nucleotide-excision repair; regulation of cell proliferation; regulation of endodeoxyribonuclease activity; regulation of gene expression, epigenetic; regulation of mitotic cell cycle; regulation of protein import into nucleus, translocation; response to DNA damage stimulus; response to hydrogen peroxide; response to insulin stimulus; response to oxidative stress; rRNA processing; single strand break repair; spermatogenesis; transcription, DNA-dependent; triacylglycerol mobilization; viral reproduction; white fat cell differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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