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SLC elisa kit :: Human SLC ELISA Kit

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Catalog # MBS2501747
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Typical Testing Data/Standard Curve (for reference only)
Product Name

SLC, ELISA Kit

Popular Item
Full Product Name

Human SLC (Secondary Lymphoid Tissue Chemokine) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602737
3D Structure
ModBase 3D Structure for O00585
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human SLC. No significant cross-reactivity or interference between Human SLC and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human SLC and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
Min: 46.875pg/mL; Max: 5000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SLC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SLCpurchase
MBS2501747 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SLC, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC. The ELISA analytical biochemical technique of the MBS2501747 kit is based on SLC antibody-SLC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SLC elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SLC in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SLC. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human SLC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SLC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SLC. You can calculate the concentration of Human SLC in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of SLC elisa kit
SLC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2501747. Click to request current manual
NCBI/Uniprot data below describe general gene information for SLC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
14,646 Da
NCBI Official Full Name
secondary lymphoid tissue chemokine
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 21
NCBI Official Symbol
CCL21  [Similar Products]
NCBI Official Synonym Symbols
ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 21; Efficient Chemoattractant for Lymphocytes; beta chemokine exodus-2; secondary lymphoid tissue chemokine; small inducible cytokine subfamily A (Cys-Cys), member 21
UniProt Protein Name
C-C motif chemokine 21
UniProt Synonym Protein Names
6Ckine; Beta-chemokine exodus-2; Secondary lymphoid-tissue chemokine; SLC; Small-inducible cytokine A21
Protein Family
UniProt Gene Name
CCL21  [Similar Products]
UniProt Synonym Gene Names
SCYA21; SLC  [Similar Products]
UniProt Entry Name
CCL21_HUMAN
NCBI Summary for SLC
This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, Sep 2014]
UniProt Comments for SLC
CCL21: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Chemokine

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine receptor binding; chemokine activity; CCR7 chemokine receptor binding

Biological Process: positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; formation of immunological synapse; positive regulation of chemotaxis; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of cell adhesion mediated by integrin; positive regulation of JNK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; positive regulation of pseudopodium formation; cell-cell signaling; positive regulation of actin filament polymerization; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; T cell costimulation; ruffle organization and biogenesis; dendritic cell chemotaxis; establishment of T cell polarity; immune response; positive regulation of cell-matrix adhesion; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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