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SLC25A4 elisa kit :: Rat solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator) , member 4 ELISA Kit

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Catalog # MBS940366
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator) , member 4 (SLC25A4), ELISA Kit

Also Known As

Rat ADP/ATP translocase 1, SLC25A4 ELISA Kit

Product Synonym Names
Rat ADP/ATP translocase 1 (SLC25A4) ELISA kit; AAC1; ANT; ANT1; PEO2; PEO3; T1; ADP/ATP translocase 1; adenine nucleotide translocator 1; adenine nucleotide translocator 1 (skeletal muscle) ; heart/skeletal muscle ATP/ADP translocator; solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator); member 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS940366 Testing Data
Sequence Length
298
3D Structure
ModBase 3D Structure for O46373
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat SLC25A4. No significant cross-reactivity or interference between rat SLC25A4 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
18.75 pg/ml-1200 pg/ml.
Sensitivity
The minimum detectable dose of rat SLC25A4 is typically less than 4.7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SLC25A4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSLC25A4purchase
MBS940366 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4 (SLC25A4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC25A4. The ELISA analytical biochemical technique of the MBS940366 kit is based on SLC25A4 antibody-SLC25A4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC25A4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC25A4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SLC25A4 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SLC25A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC25A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC25A4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC25A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SLC25A4 elisa kit
SLC25A4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS940366. Click to request current manual
NCBI/Uniprot data below describe general gene information for SLC25A4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
32,901 Da
NCBI Official Full Name
ADP/ATP translocase 1
NCBI Official Symbol
SLC25A4  [Similar Products]
NCBI Official Synonym Symbols
SLC25A5
  [Similar Products]
NCBI Protein Information
ADP/ATP translocase 1; ANT 1; ADP,ATP carrier protein 1; 30 kDa CSQ-binding protein; CSQ-binding 30 kDa protein; adenine nucleotide translocator 1; solute carrier family 25 member 4; 30 kDa calsequestrin-binding protein; solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
UniProt Protein Name
ADP/ATP translocase 1
UniProt Synonym Protein Names
30 kDa calsequestrin-binding protein; 30 kDa CSQ-binding protein; ADP,ATP carrier protein 1; Adenine nucleotide translocator 1; ANT 1; Solute carrier family 25 member 4
Protein Family
UniProt Gene Name
SLC25A4  [Similar Products]
UniProt Synonym Gene Names
ANT1; 30 kDa CSQ-binding protein; ANT 1  [Similar Products]
UniProt Entry Name
ADT1_RABIT
UniProt Comments for SLC25A4
Function: Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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