• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SLC2A4 elisa kit :: Human Glucose transporter 4 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS283942
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $745 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,405 +4 FREE 8GB USB
  10x96-Strip-Wells  /  $6,425 +8 FREE 8GB USB
SLC2A4 elisa kit
Product Name

Glucose transporter 4 (SLC2A4), ELISA Kit

Popular Item
Also Known As

Human Glucose transporter 4 (GLUT4) ELISA Kit

Product Synonym Names
GLUT4; glucose transporter 4|insulin-responsive glucose transporter type 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
M20747 mRNA
3D Structure
ModBase 3D Structure for P14672
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human SLC2A4. No significant cross-reactivity or interference between Human SLC2A4 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SLC2A4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSLC2A4purchase
MBS283942 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucose transporter 4 (SLC2A4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC2A4. The ELISA analytical biochemical technique of the MBS283942 kit is based on SLC2A4 antibody-SLC2A4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC2A4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC2A4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SLC2A4 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SLC2A4 in samples. An antibody specific for SLC2A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC2A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC2A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC2A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS283942. Click to request current manual
NCBI/Uniprot data below describe general gene information for SLC2A4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,760 Da
NCBI Official Full Name
solute carrier family 2, facilitated glucose transporter member 4
NCBI Official Synonym Full Names
solute carrier family 2 member 4
NCBI Official Symbol
SLC2A4  [Similar Products]
NCBI Official Synonym Symbols
GLUT4
  [Similar Products]
NCBI Protein Information
solute carrier family 2, facilitated glucose transporter member 4
UniProt Protein Name
Solute carrier family 2, facilitated glucose transporter member 4
UniProt Synonym Protein Names
Glucose transporter type 4, insulin-responsive; GLUT-4
Protein Family
UniProt Gene Name
SLC2A4  [Similar Products]
UniProt Synonym Gene Names
GLUT4; GLUT-4  [Similar Products]
NCBI Summary for SLC2A4
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]
UniProt Comments for SLC2A4
GLUT4: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Its surface expression is regulated by insulin. Has two internalization sequences, a dileucine repeat present in the C-terminus and a FxxY motif in the amino-terminal end. Associates with an intracellular tubulo-vesicular compartment under low plasma conditions. The binding of insulin to the insulin receptor (a typosine kinase) leads to the rapid translocation of GLUT4 to the cell surface, increasing cellular glucose transport activity. Its translocation to the plasma membrane is also stimulated by exercise, but independent from insulin signaling. This insulin-independent pathway may be regulated by AMPK.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: clathrin-coated pit; clathrin-coated vesicle; cytoplasmic vesicle membrane; cytosol; endomembrane system; external side of plasma membrane; extracellular exosome; integral component of plasma membrane; membrane; membrane raft; multivesicular body; perinuclear region of cytoplasm; plasma membrane; T-tubule; trans-Golgi network transport vesicle; vesicle membrane

Molecular Function: D-glucose transmembrane transporter activity; glucose transmembrane transporter activity; protein binding

Biological Process: amylopectin biosynthetic process; brown fat cell differentiation; carbohydrate metabolic process; cellular response to hypoxia; cellular response to insulin stimulus; cellular response to osmotic stress; cellular response to tumor necrosis factor; glucose homeostasis; glucose import; glucose import in response to insulin stimulus; glucose transmembrane transport; glucose transport; response to ethanol

Disease: Diabetes Mellitus, Noninsulin-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000