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SLC4A1 elisa kit :: Human Band 3 anion transport protein (SLC4A1) ELISA Kit

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Catalog # MBS7230564
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Typical Standard Curve/Testing Data
Product Name

Band 3 anion transport protein (SLC4A1), ELISA Kit

Popular Item
Full Product Name

Human Band 3 anion transport protein (SLC4A1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
612653
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of SLC4A1. No significant cross-reactivity or interference between SLC4A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC4A1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
25-500ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SLC4A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SLC4A1purchase
MBS7230564 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Band 3 anion transport protein (SLC4A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC4A1. The ELISA analytical biochemical technique of the MBS7230564 kit is based on SLC4A1 antibody-SLC4A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC4A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC4A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SLC4A1 elisa kit
Intended Uses: This SLC4A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SLC4A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: SLC4A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SLC4A1 antibody and an SLC4A1-HRP conjugate. The assay sample and buffer are incubated together with SLC4A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC4A1 concentration since SLC4A1 from samples and SLC4A1-HRP conjugate compete for the anti-SLC4A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC4A1 from the sample, fewer sites are left to bind SLC4A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC4A1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SLC4A1 elisa kit

Typical Standard Curve/Testing Data of SLC4A1 elisa kit
SLC4A1 elisa kit Typical Standard Curve/Testing Data image
NCBI/Uniprot data below describe general gene information for SLC4A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
101,792 Da
NCBI Official Full Name
band 3 anion transport protein
NCBI Official Synonym Full Names
solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
NCBI Official Symbol
SLC4A1  [Similar Products]
NCBI Official Synonym Symbols
DI; FR; SW; WD; WR; AE1; WD1; BND3; EPB3; CD233; EMPB3; RTA1A
  [Similar Products]
NCBI Protein Information
band 3 anion transport protein; Diego blood group; Swann blood group; anion exchanger 1; anion exchanger-1; Froese blood group; Wright blood group; Waldner blood group; anion exchange protein 1; erythroid anion exchange protein; erythrocyte membrane protein band 3; solute carrier family 4, anion exchanger, number 1; solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
UniProt Protein Name
Band 3 anion transport protein
UniProt Synonym Protein Names
Anion exchange protein 1; AE 1; Anion exchanger 1; Solute carrier family 4 member 1; CD_antigen: CD233
UniProt Gene Name
SLC4A1  [Similar Products]
UniProt Synonym Gene Names
AE1; DI; EPB3; AE 1; Anion exchanger 1  [Similar Products]
UniProt Entry Name
B3AT_HUMAN
NCBI Summary for SLC4A1
The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. [provided by RefSeq, Jul 2008]
UniProt Comments for SLC4A1
SLC4A1: Band 3 is the major integral glycoprotein of the erythrocyte membrane. Band 3 has two functional domains. Its integral domain mediates a 1:1 exchange of inorganic anions across the membrane, whereas its cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Defects in SLC4A1 are the cause of elliptocytosis type 4 (EL4). EL4 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SLC4A1 are the cause of spherocytosis type 4 (SPH4); also known as hereditary spherocytosis type 4 (HS4). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, autosomal dominant (AD-dRTA). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with hemolytic anemia (dRTA-HA). A disease characterized by the association of hemolytic anemia with distal renal tubular acidosis, the reduced ability to acidify urine resulting in variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with normal red cell morphology (dRTA-NRC). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Belongs to the anion exchanger (TC 2.A.31) family.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: cortical cytoskeleton; basolateral plasma membrane; integral to plasma membrane; plasma membrane; integral to membrane; Z disc

Molecular Function: protein C-terminus binding; bicarbonate transmembrane transporter activity; protein binding; protein homodimerization activity; protein anchor; chloride transmembrane transporter activity; inorganic anion exchanger activity; anion:anion antiporter activity; anion transmembrane transporter activity; ankyrin binding; actin binding

Biological Process: bicarbonate transport; cellular ion homeostasis; regulation of intracellular pH; ion transport; chloride transport; transmembrane transport; anion transport

Disease: Blood Group--swann System; Blood Group--froese; Renal Tubular Acidosis, Distal, Autosomal Dominant; Blood Group--wright Antigen; Renal Tubular Acidosis, Distal, With Hemolytic Anemia; Spherocytosis, Type 4; Blood Group--diego System; Malaria, Susceptibility To; Blood Group--waldner Type
Precautions
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