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SLC7A11 elisa kit :: Mouse Cystine/glutamate transporter (SLC7A11) ELISA Kit

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Catalog # MBS7216383
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cystine/glutamate transporter (SLC7A11), ELISA Kit

Popular Item
Also Known As

Mouse Cystine/glutamate transporter (SLC7A11) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
607933
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of SLC7A11. No significant cross-reactivity or interference between SLC7A11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC7A11 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This SLC7A11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SLC7A11. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SLC7A11 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSLC7A11purchase
MBS7216383 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cystine/glutamate transporter (SLC7A11) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC7A11. The ELISA analytical biochemical technique of the MBS7216383 kit is based on SLC7A11 antibody-SLC7A11 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC7A11 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC7A11. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SLC7A11 elisa kit

Principle of the assay: SLC7A11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SLC7A11 antibody and an SLC7A11-HRP conjugate. The assay sample and buffer are incubated together with SLC7A11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC7A11 concentration since SLC7A11 from samples and SLC7A11-HRP conjugate compete for the anti-SLC7A11 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC7A11 from the sample, fewer sites are left to bind SLC7A11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC7A11 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of SLC7A11 elisa kit
SLC7A11 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7216383. Click to request current manual
NCBI/Uniprot data below describe general gene information for SLC7A11. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,423 Da
NCBI Official Full Name
cystine/glutamate transporter
NCBI Official Synonym Full Names
solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
NCBI Official Symbol
SLC7A11  [Similar Products]
NCBI Official Synonym Symbols
xCT; CCBR1
  [Similar Products]
NCBI Protein Information
cystine/glutamate transporter; amino acid transport system xc-; solute carrier family 7 member 11; calcium channel blocker resistance protein CCBR1; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
UniProt Protein Name
Cystine/glutamate transporter
UniProt Synonym Protein Names
Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; Solute carrier family 7 member 11; xCT
UniProt Gene Name
SLC7A11  [Similar Products]
UniProt Entry Name
XCT_HUMAN
NCBI Summary for SLC7A11
This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death. [provided by RefSeq, Sep 2011]
UniProt Comments for SLC7A11
SLC7A11: Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 4q28.3

Cellular Component: cytoskeleton; cell surface; rough endoplasmic reticulum; integral to membrane; plasma membrane

Molecular Function: protein binding; cystine:glutamate antiporter activity

Biological Process: response to nicotine; amino acid transport; response to toxin; ion transport; response to oxidative stress; brain development; blood coagulation; leukocyte migration; transmembrane transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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