• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SMAC recombinant protein :: SMAC/Diablo Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS203157
Unit / Price
Scan QR to view Datasheet
  0.1 mg  /  $375 +1 FREE 8GB USB
  0.5 mg  /  $925 +1 FREE 8GB USB
SDS-Page
Product Name

SMAC/Diablo, Recombinant Protein

Full Product Name

SMAC/Diablo, 56-239aa, Human, T7 tag, E Coli

Product Synonym Names
Diablo isoform 1; SMAC3; DIABLO-S; FLJ10537; FLJ25049; Diablo isoform 1; SMAC/Diablo; Diablo isoform 1 0610041G12Rik; DBOH; Diablo homolog; DIABLO S; Diablo homolog (Drosophila); Mitochondrial Smac protein; Diablo homolog mitochondrial; SMAC 3; Smac protein; Direct IAP binding protein with low pI; Second mitochondria derived activator of caspase.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MASMTGGQQM GRGSMAVPIA QKSEPHSLSS EALMRRAVSL VTDSTSTFLS QTTYALIEAI TEYTKAVYTL TSLYRQYTSL LGKMNSEEED EVWQVIIGAR AEMTSKHQEY LKLETTWMTA VGLSEMAAEA AYQTGADQAS ITARNHIQLV KLQVEEVHQL SRKAETKLAE AQIEELRQKT QEEGEERAES EQEAYLRED
OMIM
AF262240 mRNA
3D Structure
ModBase 3D Structure for Q9NR28
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 20mM Tris pH 7.5
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Tag Information
T7 tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of SMAC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SMAC recombinant protein
Smac/Diablo is one of the proapoptotic proteins. Smac promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. Recombinant human Smac fused to T7-tag. at N-terminus was expressed in E Coli and purified by conventional chromatography techniques.
Product Categories/Family for SMAC recombinant protein
Applications Tested/Suitable for SMAC recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for SMAC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22 kDa (199 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
diablo homolog, mitochondrial isoform 1
NCBI Official Synonym Full Names
diablo IAP-binding mitochondrial protein
NCBI Official Symbol
DIABLO  [Similar Products]
NCBI Official Synonym Symbols
SMAC; DFNA64
  [Similar Products]
NCBI Protein Information
diablo homolog, mitochondrial
UniProt Protein Name
Diablo homolog, mitochondrial
UniProt Synonym Protein Names
Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; Smac
UniProt Gene Name
DIABLO  [Similar Products]
UniProt Synonym Gene Names
SMAC; Smac  [Similar Products]
NCBI Summary for SMAC
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
UniProt Comments for SMAC
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Product References and Citations for SMAC recombinant protein
Srinivasula SM., et al. (2000). J Biol Chem. 275 (46):36152-7.; Du C., et al. (2000). Cell. 102 (1):33-42.;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000