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SMO elisa kit :: Human smoothened homolog (Drosophila) ELISA Kit

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Catalog # MBS906870
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Typical Testing Data/Standard Curve (for reference only)
Product Name

smoothened homolog (Drosophila), ELISA Kit

Popular Item
Full Product Name

Human Smoothened homolog, SMO ELISA Kit

Product Synonym Names
Human Smoothened homolog (SMO) ELISA kit; Gx; SMOH; seven transmembrane helix receptor; smoothened; smoothened homolog (Drosophila)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS906870 Testing Data
OMIM
601500
3D Structure
ModBase 3D Structure for Q99835
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human SMO. No significant cross-reactivity or interference between human SMO and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
23.5 pg/ml-1500 pg/ml.
Sensitivity
5.8 pg /ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SMO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSMOpurchase
MBS906870 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the smoothened homolog (Drosophila) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SMO. The ELISA analytical biochemical technique of the MBS906870 kit is based on SMO antibody-SMO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SMO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SMO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SMO elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SMO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SMO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMO bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SMO elisa kit
SMO elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS906870. Click to request current manual
NCBI/Uniprot data below describe general gene information for SMO. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
86,397 Da
NCBI Official Full Name
smoothened homolog
NCBI Official Synonym Full Names
smoothened, frizzled class receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
Gx; SMOH; FZD11
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NCBI Protein Information
smoothened homolog; protein Gx; frizzled family member 11; seven transmembrane helix receptor; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor
UniProt Protein Name
Smoothened homolog
UniProt Synonym Protein Names
Protein Gx
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SMOH; SMO  [Similar Products]
UniProt Entry Name
SMO_HUMAN
NCBI Summary for SMO
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
UniProt Comments for SMO
SMO: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, integral; Cell development/differentiation; Membrane protein, multi-pass; Receptor, GPCR; GPCR, Fz/Smo family; Oncoprotein

Chromosomal Location of Human Ortholog: 7q32.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; plasma membrane; integral to membrane; caveola

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; patched binding; drug binding

Biological Process: developmental growth; central nervous system neuron differentiation; ventral midline determination; skeletal muscle fiber development; negative regulation of epithelial cell differentiation; cell fate specification; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; thalamus development; anterior/posterior pattern formation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; heart looping; vasculogenesis; activation of hh target transcription factor; neural crest cell migration; dorsoventral neural tube patterning; positive regulation of neuroblast proliferation; smoothened signaling pathway; hair follicle morphogenesis; protein stabilization; in utero embryonic development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; multicellular organism growth; smoothened signaling pathway in ventral spinal cord patterning; forebrain morphogenesis; negative regulation of DNA binding; negative regulation of hair follicle development; odontogenesis of dentine-containing teeth; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of protein import into nucleus; cerebellar cortex morphogenesis; myoblast migration; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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