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SOD3 elisa kit :: Human Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit

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Catalog # MBS2023115 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $255 +1 FREE 8GB USB
  48-Strip-Wells  /  $455 +1 FREE 8GB USB
  96-Strip-Wells  /  $610 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Superoxide Dismutase 3, Extracellular (SOD3), ELISA Kit

Popular Item
Also Known As

Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit

Product Synonym Names
SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
240
OMIM
185490
3D Structure
ModBase 3D Structure for P08294
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 3, Extracellular (SOD3).
No significant cross-reactivity or interference between Superoxide Dismutase 3, Extracellular (SOD3) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Cell lysates and Other Biological Fluids
Detection Range
125-8000pg/mL
Sensitivity
< 55pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SOD3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSOD3purchase
MBS2023115 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SOD3. The ELISA analytical biochemical technique of the MBS2023115 kit is based on SOD3 antibody-SOD3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SOD3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SOD3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SOD3 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 3, Extracellular (SOD3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase 3, Extracellular (SOD3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for SOD3 elisa kit

Typical Testing Data/Standard Curve (for reference only) of SOD3 elisa kit
SOD3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2023115. Click to request current manual
NCBI/Uniprot data below describe general gene information for SOD3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,851 Da
NCBI Official Full Name
superoxide dismutase 3, extracellular
NCBI Official Synonym Full Names
superoxide dismutase 3, extracellular
NCBI Official Symbol
NCBI Official Synonym Symbols
EC-SOD
  [Similar Products]
NCBI Protein Information
extracellular superoxide dismutase [Cu-Zn]
UniProt Protein Name
Extracellular superoxide dismutase [Cu-Zn]
UniProt Gene Name
UniProt Synonym Gene Names
EC-SOD  [Similar Products]
UniProt Entry Name
SODE_HUMAN
NCBI Summary for SOD3
This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]
UniProt Comments for SOD3
SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Secreted, signal peptide; Secreted; EC 1.15.1.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus

Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity

Biological Process: response to reactive oxygen species; removal of superoxide radicals; response to copper ion; response to hypoxia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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