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SPD elisa kit :: Human Surfactant Associated Protein D (SPD) ELISA Kit

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Catalog # MBS266801
Unit / Price
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  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Surfactant Associated Protein D (SPD), ELISA Kit

Popular Item
Full Product Name

Human Surfactant Associated Protein D (SPD)

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
No cross-reaction with other factors.
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SPD concentration
Assay Type
Detection Range
200 ng/ml-3.12 ng/ml
0.6 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SPD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SPDpurchase
MBS266801 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Surfactant Associated Protein D (SPD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPD. The ELISA analytical biochemical technique of the MBS266801 kit is based on SPD antibody-SPD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SPD elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SPD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of SPD elisa kit
SPD elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS266801. Click to request current manual
NCBI/Uniprot data below describe general gene information for SPD. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Related Accession #
Molecular Weight
37,688 Da
NCBI Official Full Name
Surfactant associated protein D
NCBI Official Synonym Full Names
surfactant associated protein D
NCBI Official Symbol
Sftpd  [Similar Products]
NCBI Official Synonym Symbols
SP-D; Sftp4; AI573415
  [Similar Products]
NCBI Protein Information
pulmonary surfactant-associated protein D; PSP-D; lung surfactant protein D
UniProt Protein Name
Pulmonary surfactant-associated protein D
UniProt Synonym Protein Names
Lung surfactant protein D
UniProt Gene Name
Sftpd  [Similar Products]
UniProt Synonym Gene Names
Sftp4; PSP-D; SP-D  [Similar Products]
UniProt Entry Name
UniProt Comments for SPD
SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: multivesicular body; extracellular space; proteinaceous extracellular matrix; collagen; rough endoplasmic reticulum; cytoplasm; extracellular region; cytoplasmic vesicle

Molecular Function: monosaccharide binding; identical protein binding; lipopolysaccharide binding; carbohydrate binding

Biological Process: negative regulation of phagocytosis; negative regulation of T cell proliferation; immune system process; positive regulation of phagocytosis; innate immune response; respiratory gaseous exchange; opsonization; regulation of liquid surface tension; surfactant homeostasis; alveolus development; negative regulation of interleukin-2 production
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with SPD elisa kit
Organs/Tissues associated with SPD elisa kit
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