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SPHK1 elisa kit :: Human Sphingosine Kinase 1 (SPHK1) ELISA Kit

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Catalog # MBS2024588 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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Typical Standard Curve/Testing Data
Product Name

Sphingosine Kinase 1 (SPHK1), ELISA Kit

Popular Item
Full Product Name

Sphingosine Kinase 1 (SPHK1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for Q8L7L1
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of Sphingosine Kinase 1 (SPHK1).
No significant cross-reactivity or interference between Sphingosine Kinase 1 (SPHK1) and analogues was observed.
Assay Type
Double-antibody Sandwich
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
< 0.129ng/mL
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Sphingosine Kinase 1 (SPHK1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Sphingosine Kinase 1 (SPHK1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SPHK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SPHK1purchase
MBS2024588 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sphingosine Kinase 1 (SPHK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPHK1. The ELISA analytical biochemical technique of the MBS2024588 kit is based on SPHK1 antibody-SPHK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPHK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPHK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SPHK1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine Kinase 1 (SPHK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine Kinase 1 (SPHK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine Kinase 1 (SPHK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine Kinase 1 (SPHK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for SPHK1 elisa kit

Typical Standard Curve/Testing Data of SPHK1 elisa kit
SPHK1 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS2024588. Click to request current manual
NCBI/Uniprot data below describe general gene information for SPHK1. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,548 Da
NCBI Official Full Name
sphingosine kinase 1
NCBI Official Symbol
SPHK1  [Similar Products]
NCBI Official Synonym Symbols
F18E5.160; F18E5_160; sphingosine kinase 1; SPHK1
  [Similar Products]
NCBI Protein Information
sphingosine kinase 1
UniProt Protein Name
Sphingosine kinase 1
UniProt Gene Name
SPHK1  [Similar Products]
UniProt Entry Name
NCBI Summary for SPHK1
Encodes a sphingosine kinase, also has enzyme activity towards other plant long-chain sphingoid bases. Involved in guard cell ABA signalling and seed germination.
UniProt Comments for SPHK1
Involved in the production of sphingolipid metabolites. Phosphorylates sphingosine and various sphingoid long-chain base (LCB) products, such as phytosphingosine (PHS, 4-hydroxysphinganine), 4-hydroxy-8-sphingenine, 4,8-sphingadienine, D-erythro-dihydrosphingosine and D,L-threo-dihydrosphingosine. Is required for abscisic acid (ABA) signaling that mediates stomatal closure, inhibition of seed germination and root elongation. May function upstream of PLDALPHA1 and phosphatidic acid (PA) in an amplification response to ABA that mediates stomatal closure.
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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