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Sphk1 elisa kit :: Mouse Sphingosine kinase 1 ELISA Kit

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Catalog # MBS288761
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  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Sphk1 elisa kit
Product Name

Sphingosine kinase 1 (Sphk1), ELISA Kit

Popular Item
Full Product Name

Mouse Sphingosine kinase 1 ELISA Kit

Product Synonym Names
Sphingosine kinase 1; SK 1; SPK 1; Sphk1; 2.7.1.91
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q8CI15
Species Reactivity
Assay Type
Sandwich
Detection Range
31.2-2000 pg/ml
Sensitivity
15.75 pg/mL
Intra-Assay CV
<=5.9%
Inter-Assay CV
<=8.3%
Recovery
107%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Sphk1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Sphk1purchase
MBS288761 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sphingosine kinase 1 (Sphk1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Sphk1. The ELISA analytical biochemical technique of the MBS288761 kit is based on Sphk1 antibody-Sphk1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Sphk1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Sphk1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Sphk1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS288761. Click to request current manual
NCBI/Uniprot data below describe general gene information for Sphk1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,344 Da
NCBI Official Full Name
sphingosine kinase 1 isoform 2
NCBI Official Synonym Full Names
sphingosine kinase 1
NCBI Official Symbol
Sphk1  [Similar Products]
NCBI Official Synonym Symbols
Sk1; Spk1; 1110006G24Rik
  [Similar Products]
NCBI Protein Information
sphingosine kinase 1
UniProt Protein Name
Sphingosine kinase 1
Protein Family
UniProt Gene Name
Sphk1  [Similar Products]
UniProt Synonym Gene Names
SK 1; SPK 1  [Similar Products]
UniProt Entry Name
SPHK1_MOUSE
NCBI Summary for Sphk1
This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
UniProt Comments for Sphk1
SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91

Cellular Component: axon; cytoplasm; cytosol; membrane; neuron projection; nucleus; plasma membrane; synaptic vesicle

Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; kinase activity; magnesium ion binding; nucleotide binding; protein phosphatase 2A binding; sphinganine kinase activity; transferase activity

Biological Process: activation of NF-kappaB transcription factor; blood vessel development; brain development; calcium-mediated signaling; cyclooxygenase pathway; inflammatory response; lipid phosphorylation; negative regulation of apoptosis; phosphorylation; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; positive regulation of NF-kappaB import into nucleus; positive regulation of protein amino acid phosphorylation; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; regulation of interleukin-1 beta production; sphingosine biosynthetic process; sphingosine metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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