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SPHK2 peptide :: Sphingosine Kinase 2, Long Form, Human Peptide

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Catalog # MBS396454
Unit / Price
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  0.05 mg  /  $205 +1 FREE 8GB USB
SPHK2 peptide
Product Name

Sphingosine Kinase 2, Long Form, Human (SPHK2), Peptide

Popular Item
Full Product Name

Sphingosine Kinase 2, Long Form, Human

Product Synonym Names
Sphk2-L; N-terminal-extended Sphingosine Kinase 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 19; NC_000019.9 (49122548..49133663). Location: 19q13.2
OMIM
607092
3D Structure
ModBase 3D Structure for Q9NRA0
Isotype
IgG
Host
Rabbit
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Comments
Blocking peptide for use with Human Sphingosine Kinase 2, Long Form antibodies.
Instruction
Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37 Deg. C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western Blot.

Example: 10ul or 10ug of ExalphaÂ’s rabbit anti-human Sphingosine Kinase 2 , Long form is added to 10ug of blocking peptide for a total volume of 20 ul. The mixture is allowed to incubate for 20 minutes at 37 Deg. C prior to dilution in suitable buffer (for Western Blot, etc.).
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Products are stable for one year from purchase when stored properly.
Other Notes
Small volumes of SPHK2 peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SPHK2 peptide
Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.
Product Categories/Family for SPHK2 peptide
Applications Tested/Suitable for SPHK2 peptide
Antibody Function Blocking
NCBI/Uniprot data below describe general gene information for SPHK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69,217 Da[Similar Products]
NCBI Official Full Name
sphingosine kinase 2 isoform a
NCBI Official Synonym Full Names
sphingosine kinase 2
NCBI Official Symbol
SPHK2  [Similar Products]
NCBI Official Synonym Symbols
SK 2; SK-2; SPK 2; SPK-2
  [Similar Products]
NCBI Protein Information
sphingosine kinase 2; sphingosine kinase type 2
UniProt Protein Name
Sphingosine kinase 2
Protein Family
UniProt Gene Name
SPHK2  [Similar Products]
UniProt Synonym Gene Names
SK 2; SPK 2  [Similar Products]
UniProt Entry Name
SPHK2_HUMAN
NCBI Summary for SPHK2
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
UniProt Comments for SPHK2
Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. Ref.8

Catalytic activity: ATP + sphinganine = ADP + sphinganine 1-phosphate. Ref.1ATP + sphingosine = ADP + sphingosine 1-phosphate. Ref.1

Cofactor: Magnesium

By similarity.

Enzyme regulation: Inhibited by sulfatide. Ref.8

Subcellular location: Isoform 1: Cytoplasm. Membrane Ref.6 Ref.8. Isoform 2: Lysosome membrane Ref.6 Ref.8.

Sequence similarities: Contains 1 DAGKc domain.

Biophysicochemical propertiesKinetic parameters:KM=3.4 µM for sphingosine Ref.1pH dependence:Optimum pH is 7.5.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with SPHK2 peptide
 Reference Product  PubMed Publications
 SGPP1 peptide  >2 publications with SPHK2 and SGPP1
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