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SPINK7 elisa kit :: Human serine peptidase inhibitor, Kazal type 7 (putative) ELISA Kit

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Catalog # MBS945133
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Typical Testing Data/Standard Curve (for reference only)
Product Name

serine peptidase inhibitor, Kazal type 7 (putative) (SPINK7), ELISA Kit

Popular Item
Full Product Name

Human Serine protease inhibitor Kazal-type 7, SPINK7 ELISA Kit

Product Synonym Names
Human Serine protease inhibitor Kazal-type 7 (SPINK7) ELISA kit; ECG2; ECRG2; MGC133105; MGC133106; esophagus cancer related gene 2; esophagus cancer-related gene-2; serine peptidase inhibitor; Kazal type 7 (putative)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS945133 Testing Data
3D Structure
ModBase 3D Structure for P58062
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human SPINK7. No significant cross-reactivity or interference between human SPINK7 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0. 156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of human SPINK7 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SPINK7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SPINK7purchase
MBS945133 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the serine peptidase inhibitor, Kazal type 7 (putative) (SPINK7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPINK7. The ELISA analytical biochemical technique of the MBS945133 kit is based on SPINK7 antibody-SPINK7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPINK7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPINK7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SPINK7 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPINK7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPINK7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINK7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINK7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SPINK7 elisa kit
SPINK7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS945133. Click to request current manual
NCBI/Uniprot data below describe general gene information for SPINK7. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
9,232 Da
NCBI Official Full Name
serine protease inhibitor Kazal-type 7
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kazal type 7 (putative)
NCBI Official Symbol
SPINK7  [Similar Products]
NCBI Official Synonym Symbols
ECG2; ECRG2
  [Similar Products]
NCBI Protein Information
serine protease inhibitor Kazal-type 7; ECRG-2; esophagus cancer related gene 2; esophagus cancer-related gene-2; esophagus cancer-related gene 2 protein
UniProt Protein Name
Serine protease inhibitor Kazal-type 7
UniProt Synonym Protein Names
Esophagus cancer-related gene 2 protein; ECRG-2
Protein Family
UniProt Gene Name
SPINK7  [Similar Products]
UniProt Synonym Gene Names
ECG2; UNQ745/PRO1474; ECRG-2  [Similar Products]
UniProt Entry Name
ISK7_HUMAN
UniProt Comments for SPINK7
SPINK7: Probable serine protease inhibitor.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with SPINK7 elisa kit
 Reference Product  PubMed Publications
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