• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SPTAN1 elisa kit :: Human spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS911505
Unit / Price
Scan QR to view Datasheet
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) (SPTAN1), ELISA Kit

Full Product Name

Human Spectrin alpha chain, brain, SPTAN1 ELISA Kit

Product Synonym Names
Human Spectrin alpha chain; brain (SPTAN1) ELISA kit; (ALPHA) II-SPECTRIN; FLJ44613; NEAS; non-erythrocytic spectrin alpha; spectrin; alpha; non-erythrocytic 1 (alpha-fodrin)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 613477
3D Structure
ModBase 3D Structure for Q13813
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human SPTAN1. No significant cross-reactivity or interference between Human SPTAN1 and analogues was observed.
Assay Type
Sandwich
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SPTAN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SPTAN1purchase
MBS911505 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) (SPTAN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPTAN1. The ELISA analytical biochemical technique of the MBS911505 kit is based on SPTAN1 antibody-SPTAN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPTAN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPTAN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SPTAN1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPTAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPTAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTAN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SPTAN1 elisa kit
SPTAN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for SPTAN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
284,539 Da
NCBI Official Full Name
spectrin alpha chain, non-erythrocytic 1 isoform 1
NCBI Official Synonym Full Names
spectrin, alpha, non-erythrocytic 1
NCBI Official Symbol
SPTAN1  [Similar Products]
NCBI Official Synonym Symbols
NEAS; EIEE5; SPTA2
  [Similar Products]
NCBI Protein Information
spectrin alpha chain, non-erythrocytic 1; alpha-fodrin; alpha-II spectrin; fodrin alpha chain; spectrin, non-erythroid alpha chain; spectrin, non-erythroid alpha subunit
UniProt Protein Name
Spectrin alpha chain, non-erythrocytic 1
UniProt Synonym Protein Names
Alpha-II spectrin; Fodrin alpha chain; Spectrin, non-erythroid alpha subunit
Protein Family
UniProt Gene Name
SPTAN1  [Similar Products]
UniProt Synonym Gene Names
NEAS; SPTA2  [Similar Products]
UniProt Entry Name
SPTN1_HUMAN
NCBI Summary for SPTAN1
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
UniProt Comments for SPTAN1
SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: microtubule cytoskeleton; spectrin; membrane; intracellular membrane-bound organelle; fascia adherens; cytosol; Z disc; vesicle; lateral plasma membrane

Molecular Function: calmodulin binding; protein binding; spectrin binding; structural constituent of cytoskeleton; protein heterodimerization activity; calcium ion binding; actin binding

Biological Process: axon guidance; apoptosis; actin filament capping; cell structure disassembly during apoptosis

Disease: Epileptic Encephalopathy, Early Infantile, 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with SPTAN1 elisa kit
 Reference Product  PubMed Publications
 SPTBN1 elisa kit  >1 publications with SPTAN1 and SPTBN1
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000