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SRD5A1 elisa kit :: Canine 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) ELISA Kit

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Catalog # MBS7224403
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1), ELISA Kit

Full Product Name

Canine 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
184753
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of SRD5A1. No significant cross-reactivity or interference between SRD5A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SRD5A1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Intended Uses
This SRD5A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine SRD5A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SRD5A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SRD5A1purchase
MBS7224403 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SRD5A1. The ELISA analytical biochemical technique of the MBS7224403 kit is based on SRD5A1 antibody-SRD5A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SRD5A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SRD5A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SRD5A1 elisa kit
Principle of the assay: SRD5A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SRD5A1 antibody and an SRD5A1-HRP conjugate. The assay sample and buffer are incubated together with SRD5A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRD5A1 concentration since SRD5A1 from samples and SRD5A1-HRP conjugate compete for the anti-SRD5A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SRD5A1 from the sample, fewer sites are left to bind SRD5A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRD5A1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SRD5A1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of SRD5A1 elisa kit
SRD5A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7224403. Click to request current manual
NCBI/Uniprot data below describe general gene information for SRD5A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,459 Da
NCBI Official Full Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
NCBI Official Synonym Full Names
steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
NCBI Official Symbol
SRD5A1  [Similar Products]
NCBI Official Synonym Symbols
S5AR 1
  [Similar Products]
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; 5-alpha reductase; steroid 5-alpha-reductase 1; steroid 5-alpha-reductase type I; 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1
UniProt Protein Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
UniProt Synonym Protein Names
SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
UniProt Gene Name
SRD5A1  [Similar Products]
UniProt Synonym Gene Names
S5AR 1  [Similar Products]
UniProt Entry Name
S5A1_HUMAN
NCBI Summary for SRD5A1
Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). Also see SRD5A2 (MIM 607306).[supplied by OMIM, Mar 2008]
UniProt Comments for SRD5A1
SRD5A1: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5- alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Belongs to the steroid 5-alpha reductase family.

Protein type: EC 1.3.1.22; Membrane protein, multi-pass; Oxidoreductase; Lipid Metabolism - androgen and estrogen; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: electron carrier activity; cholestenone 5-alpha-reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity

Biological Process: sex determination; steroid metabolic process; androgen biosynthetic process; cell differentiation; sex differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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