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SREBF1 elisa kit :: Human sterol regulatory element binding transcription factor 1 ELISA Kit

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Catalog # MBS916923
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Typical Testing Data/Standard Curve (for reference only)
Product Name

sterol regulatory element binding transcription factor 1 (SREBF1), ELISA Kit

Popular Item
Full Product Name

Human Sterol regulatory element-binding protein 1, SREBF1 ELISA Kit

Product Synonym Names
Human Sterol regulatory element-binding protein 1 (SREBF1) ELISA kit; SREBP-1c; SREBP1; bHLHd1; sterol regulatory element binding protein-1; sterol regulatory element binding transcription factor 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS916923 Testing Data
OMIM
184756
3D Structure
ModBase 3D Structure for P36956
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human SREBF1. No significant cross-reactivity or interference between human SREBF1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
7.8 pg/ml -500 pg/ml
Sensitivity
1.95 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SREBF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SREBF1purchase
MBS916923 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sterol regulatory element binding transcription factor 1 (SREBF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SREBF1. The ELISA analytical biochemical technique of the MBS916923 kit is based on SREBF1 antibody-SREBF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SREBF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SREBF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SREBF1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SREBF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SREBF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SREBF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SREBF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SREBF1 elisa kit
SREBF1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS916923. Click to request current manual
NCBI/Uniprot data below describe general gene information for SREBF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
121,675 Da
NCBI Official Full Name
sterol regulatory element-binding protein 1 isoform a
NCBI Official Synonym Full Names
sterol regulatory element binding transcription factor 1
NCBI Official Symbol
SREBF1  [Similar Products]
NCBI Official Synonym Symbols
SREBP1; bHLHd1; SREBP-1c
  [Similar Products]
NCBI Protein Information
sterol regulatory element-binding protein 1; SREBP-1; class D basic helix-loop-helix protein 1
UniProt Protein Name
Sterol regulatory element-binding protein 1
UniProt Synonym Protein Names
Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1Cleaved into the following chain:Processed sterol regulatory element-binding protein 1
UniProt Gene Name
SREBF1  [Similar Products]
UniProt Synonym Gene Names
BHLHD1; SREBP1; SREBP-1; bHLHd1  [Similar Products]
UniProt Entry Name
SRBP1_HUMAN
NCBI Summary for SREBF1
This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SREBF1
SREBP-1: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. Belongs to the SREBP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cytoplasm; integral to membrane; nuclear envelope; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; sterol response element binding; DNA binding; sequence-specific DNA binding; protein complex binding; chromatin binding; transcription factor activity; protein kinase binding

Biological Process: circadian rhythm; response to cAMP; response to glucagon stimulus; lipid biosynthetic process; positive regulation of cholesterol biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; negative regulation of insulin secretion; positive regulation of histone deacetylation; regulation of fatty acid metabolic process; response to glucose stimulus; aging; response to drug; response to food; cholesterol metabolic process; response to retinoic acid; transcription, DNA-dependent; cellular response to starvation; regulation of transcription from RNA polymerase II promoter; chemical signal regulation of heart rate; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; response to progesterone stimulus; lung development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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