• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SRGN elisa kit :: Human serglycin ELISA Kit

Scan QR to view Datasheet
Catalog # MBS910006
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

serglycin (SRGN), ELISA Kit

Popular Item
Also Known As

Human Serglycin, SRGN ELISA Kit

Product Synonym Names
Human Serglycin (SRGN) ELISA kit; FLJ12930; MGC9289; PPG; PRG; PRG1; hematopoetic proteoglycan core peptide; platelet proteoglycan protein core; proteoglycan 1; secretory granule; proteoglycan protein core for mast cell secretory granu; serglycin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS910006 Testing Data
Sequence Length
158
OMIM
177040
3D Structure
ModBase 3D Structure for P10124
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human SRGN. No significant cross-reactivity or interference between human SRGN and analogues was observed.
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
0.312 ng/ml -20 ng/ml.
Sensitivity
0.078 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SRGN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSRGNpurchase
MBS910006 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the serglycin (SRGN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SRGN. The ELISA analytical biochemical technique of the MBS910006 kit is based on SRGN antibody-SRGN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SRGN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SRGN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SRGN elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SRGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SRGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRGN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRGN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SRGN elisa kit
SRGN elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS910006. Click to request current manual
NCBI/Uniprot data below describe general gene information for SRGN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,652 Da
NCBI Official Full Name
serglycin
NCBI Official Synonym Full Names
serglycin
NCBI Official Symbol
NCBI Official Synonym Symbols
PPG; PRG; PRG1
  [Similar Products]
NCBI Protein Information
serglycin; p.PG; serglycin proteoglycan; proteoglycan 1, secretory granule; platelet proteoglycan core protein; hematopoetic proteoglycan core peptide; hematopoetic proteoglycan core protein; hematopoietic proteoglycan core protein; secretory granule proteoglycan core peptide; secretory granule proteoglycan core protein; proteoglycan protein core for mast cell secretory granule
UniProt Protein Name
Serglycin
UniProt Synonym Protein Names
Hematopoietic proteoglycan core protein; Platelet proteoglycan core protein; P.PG; Secretory granule proteoglycan core protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PRG; PRG1; P.PG  [Similar Products]
UniProt Entry Name
SRGN_HUMAN
NCBI Summary for SRGN
This gene encodes a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This encoded protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Comments for SRGN
SRGN: Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF- alpha and may also regulate protease secretion. Inhibits bone mineralization. Belongs to the serglycin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; mast cell granule; extracellular region

Molecular Function: collagen binding; protein binding

Biological Process: maintenance of granzyme B localization in T cell secretory granule; negative regulation of bone mineralization; platelet activation; platelet degranulation; induction of apoptosis by granzyme; biomineral formation; negative regulation of cytokine secretion; T cell secretory granule organization and biogenesis; protein processing; maintenance of protease localization in mast cell secretory granule; mast cell secretory granule organization and biogenesis; blood coagulation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withSRGN elisa kit
 Reference Product  PubMed Publications
 CLU elisa kit  >1 publications with SRGN and CLU
Organs/Tissues associated with SRGN elisa kit
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000