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Sry recombinant protein :: Sex-determining region Y protein Recombinant Protein

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Catalog # MBS963769
Unit / Price
  0.01 mg (E-Coli)  /  $110 +1 FREE 8GB USB
  0.01 mg (Yeast)  /  $110 +1 FREE 8GB USB
  0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $190 +1 FREE 8GB USB
  0.1 mg (E-Coli)  /  $285 +1 FREE 8GB USB
  0.1 mg (Yeast)  /  $285 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $460 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $750 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $855 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,075 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,090 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
  1 mg (Yeast)  /  $1,180 +1 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $1,560 +1 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $1,665 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $2,085 +2 FREE 8GB USB
SDS-PAGE
Product Name

Sex-determining region Y protein (Sry), Recombinant Protein

Popular Item
Also Known As

Recombinant Mouse Sex-determining region Y protein

Product Synonym Names
Testis-determining factor
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-144aa; Partial
Sequence Length
395
Sequence
MEGHVKRPMN AFMVWSRGER HKLAQQNPSM QNTEISKQLG CRWKSLTEAE KRPFFQEAQR LKILHREKYP NYKYQPHRRA KVSQRSGILQ PAVASTKLYN LLQWDRNPHA ITYRQDWSRA AHLYSKNQQS FYWQPVDIPT GHLQ
3D Structure
ModBase 3D Structure for Q05738
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of Sry recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Sry recombinant protein
Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Promotes DNA bending. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. 1 Publication

Sry recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for Sry. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
21.3kD
NCBI Official Full Name
sex-determining region Y protein
NCBI Official Synonym Full Names
sex determining region of Chr Y
NCBI Official Symbol
NCBI Official Synonym Symbols
Tdf; Tdy
  [Similar Products]
NCBI Protein Information
sex-determining region Y protein
UniProt Protein Name
Sex-determining region Y protein
UniProt Synonym Protein Names
Testis-determining factor
UniProt Gene Name
UniProt Synonym Gene Names
Tdf; Tdy  [Similar Products]
UniProt Entry Name
SRY_MOUSE
UniProt Comments for Sry
SRY: Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. Defects in SRY are the cause of 46,XY sex reversal type 1 (SRXY1). A condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Patients manifest rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, streak gonads, variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female. Defects in SRY are the cause of 46,XX sex reversal type 1 (SRXX1). A condition in which male gonads develop in a genetic female (female to male sex reversal). Belongs to the SRY family.

Protein type: DNA-binding; Nuclear receptor co-regulator

Cellular Component: cytoplasm; nucleus

Molecular Function: calmodulin binding; DNA bending activity; DNA binding; protein binding; protein heterodimerization activity; transcription factor activity

Biological Process: cell differentiation; male gonad development; male sex determination; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; sex determination; sex differentiation; transcription, DNA-dependent
Product References and Citations for Sry recombinant protein
Inverted repeat structure of the Sry locus in mice.Gubbay J., Vivian N., Economou A., Jackson D., Goodfellow P.Proc. Natl. Acad. Sci. U.S.A. 89:7953-7957(1992) Rapid evolution of the sex determining locus in Old World mice and rats.Tucker P.K., Lundrigan B.L.Nature 364:715-717(1993) Polymorphism of a CAG trinucleotide repeat within Sry correlates with B6.YDom sex reversal.Coward P., Nagai K., Chen D., Thomas H.D., Nagamine C.M., Lau Y.-F.C.Nat. Genet. 6:245-250(1994) A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes.Gubbay J., Collignon J., Koopman P., Capel B., Economou A., Munsterberg A., Vivian N., Goodfellow P., Lovell-Badge R.Nature 346:245-250(1990) Definition of a consensus DNA binding site for SRY.Harley V.R., Lovell-Badge R., Goodfellow P.N.Nucleic Acids Res. 22:1500-1501(1994) Distinct DNA-binding properties of the high mobility group domain of murine and human SRY sex-determining factors.Giese K., Pagel J., Grosschedl R.Proc. Natl. Acad. Sci. U.S.A. 91:3368-3372(1994) Expression of Sry, the mouse sex determining gene.Hacker A., Capel B., Goodfellow P., Lovell-Badge R.Development 121:1603-1614(1995) Expression of a linear Sry transcript in the mouse genital ridge.Jeske Y.W., Bowles J., Greenfield A., Koopman P.Nat. Genet. 10:480-482(1995) Developmental profile of Sry transcripts in mouse brain.Mayer A., Mosler G., Just W., Pilgrim C., Reisert I.Neurogenetics 3:25-30(2000) Evidence that Sry is expressed in pre-Sertoli cells and Sertoli and granulosa cells have a common precursor.Albrecht K.H., Eicher E.M.Dev. Biol. 240:92-107(2001) The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1.Forwood J.K., Harley V., Jans D.A.J. Biol. Chem. 276:46575-46582(2001) Sry-directed sex reversal in transgenic mice is robust with respect to enhanced DNA bending comparison of human and murine HMG boxes.Phillips N.B., Nikolskaya T., Jancso-Radek A., Ittah V., Jiang F., Singh R., Haas E., Weiss M.A.Biochemistry 43:7066-7081(2004) Regulation of human SRY subcellular distribution by its acetylation/deacetylation.Thevenet L., Mejean C., Moniot B., Bonneaud N., Galeotti N., Aldrian-Herrada G., Poulat F., Berta P., Benkirane M., Boizet-Bonhoure B.EMBO J. 23:3336-3345(2004) Sry associates with the heterochromatin protein 1 complex by interacting with a KRAB domain protein.Oh H.J., Li Y., Lau Y.-F.C.Biol. Reprod. 72:407-415(2005) NHERF2/SIP-1 interacts with mouse SRY via a different mechanism than human SRY.Thevenet L., Albrecht K.H., Malki S., Berta P., Boizet-Bonhoure B., Poulat F.J. Biol. Chem. 280:38625-38630(2005) Direct regulation of adult brain function by the male-specific factor SRY.Dewing P., Chiang C.W., Sinchak K., Sim H., Fernagut P.-O., Kelly S., Chesselet M.-F., Micevych P.E., Albrecht K.H., Harley V.R., Vilain E.Curr. Biol. 16:415-420(2006) The poly(ADP-ribose) polymerase 1 interacts with Sry and modulates its biological functions.Li Y., Oh H.J., Lau Y.-F.C.Mol. Cell. Endocrinol. 257:35-46(2006) Sry and the hesitant beginnings of male development.Polanco J.C., Koopman P.Dev. Biol. 302:13-24(2007) KRAB a partner for SRY action on chromatin.Oh H.J., Lau Y.F.Mol. Cell. Endocrinol. 247:47-52(2006)

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