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StAR elisa kit :: Rat Steroidogenic acute regulatory protein ELISA Kit

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Catalog # MBS729454
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Steroidogenic acute regulatory protein (StAR), ELISA Kit

Popular Item
Full Product Name

Rat Steroidogenic acute regulatory protein ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of STAR. No significant cross-reactivity or interference between STAR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STAR and all the analogues, therefore, cross reaction may still exist in some cases.
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of StAR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forStARpurchase
MBS729454 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Steroidogenic acute regulatory protein (StAR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing StAR. The ELISA analytical biochemical technique of the MBS729454 kit is based on StAR antibody-StAR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect StAR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, StAR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
StAR elisa kit
Intended Uses: This STAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat STAR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: STAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-STAR antibody and an STAR-HRP conjugate. The assay sample and buffer are incubated together with STAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STAR concentration since STAR from samples and STAR-HRP conjugate compete for the anti-STAR antibody binding site. Since the number of sites is limited, as more sites are occupied by STAR from the sample, fewer sites are left to bind STAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAR concentration in each sample is interpolated from this standard curve.
Product Categories/Family for StAR elisa kit

Typical Testing Data/Standard Curve (for reference only) of StAR elisa kit
StAR elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS729454. Click to request current manual
NCBI/Uniprot data below describe general gene information for StAR. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,914 Da
NCBI Official Full Name
steroidogenic acute regulatory protein
NCBI Official Synonym Full Names
steroidogenic acute regulatory protein
NCBI Official Symbol
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
steroidogenic acute regulatory protein, mitochondrial; cholesterol trafficker; START domain containing 1; steroid acute regulatory protein; START domain-containing protein 1; mitochondrial steroid acute regulatory protein; StAR-related lipid transfer (START) domain containing 1
UniProt Protein Name
Steroidogenic acute regulatory protein, mitochondrial
UniProt Synonym Protein Names
START domain-containing protein 1; StARD1
UniProt Gene Name
UniProt Synonym Gene Names
STARD1; StAR; StARD1  [Similar Products]
UniProt Entry Name
NCBI Summary for StAR
The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]
UniProt Comments for StAR
STAR: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. May interact with TSPO. Expressed in gonads, adrenal cortex and kidney.

Protein type: Mitochondrial; Carrier; Lipid-binding

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: mitochondrial crista; neuron projection; cell soma; mitochondrial intermembrane space; cytosol

Molecular Function: cholesterol transporter activity; cholesterol binding

Biological Process: response to nicotine; steroid metabolic process; diterpenoid metabolic process; dibenzo-p-dioxin metabolic process; regulation of steroid biosynthetic process; estrogen biosynthetic process; biphenyl metabolic process; circadian sleep/wake cycle, REM sleep; negative regulation of neuron apoptosis; response to nutrient; response to corticosterone stimulus; response to drug; bile acid biosynthetic process; cholesterol metabolic process; positive regulation of neurogenesis; progesterone biosynthetic process; insecticide metabolic process; fractalkine metabolic process; male gonad development; phthalate metabolic process; intracellular cholesterol transport; response to herbicide; glucocorticoid metabolic process; response to ethanol; cellular response to insulin stimulus; response to hydrogen peroxide; response to estrogen stimulus; response to lead ion; C21-steroid hormone biosynthetic process; response to activity; response to ionizing radiation; brain development; steroid biosynthetic process; phenol metabolic process; regulation of neuronal synaptic plasticity

Disease: Lipoid Congenital Adrenal Hyperplasia
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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