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STAT2 elisa kit :: Mouse STAT2 ELISA Kit

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Catalog # MBS2504928
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

STAT2, ELISA Kit

Popular Item
Full Product Name

Mouse STAT2 (Signal Transducer And Activator Of Transcription 2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600556
3D Structure
ModBase 3D Structure for P52630
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse STAT2. No significant cross-reactivity or interference between Mouse STAT2 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
Min: 0.375ng/mL; Max: 40ng/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Mouse STAT2 concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of STAT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for STAT2purchase
MBS2504928 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the STAT2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing STAT2. The ELISA analytical biochemical technique of the MBS2504928 kit is based on STAT2 antibody-STAT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect STAT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, STAT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
STAT2 elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to STAT2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for STAT2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain STAT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of STAT2. You can calculate the concentration of STAT2 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of STAT2 elisa kit
STAT2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2504928. Click to request current manual
NCBI/Uniprot data below describe general gene information for STAT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
97,457 Da
NCBI Official Full Name
Stat2
NCBI Official Synonym Full Names
signal transducer and activator of transcription 2, 113kDa
NCBI Official Symbol
STAT2  [Similar Products]
NCBI Official Synonym Symbols
P113; ISGF-3; STAT113
  [Similar Products]
NCBI Protein Information
signal transducer and activator of transcription 2; interferon alpha induced transcriptional activator; signal transducer and activator of transcription 2, 113kD
UniProt Protein Name
Signal transducer and activator of transcription 2
UniProt Synonym Protein Names
p113
UniProt Gene Name
STAT2  [Similar Products]
UniProt Entry Name
STAT2_HUMAN
NCBI Summary for STAT2
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Comments for STAT2
STAT2: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Binds to the IFN-stimulated response element (ISRE) and to the Gas element. This multiprotein transcription factor is termed ISGF3. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; signal transducer activity; DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; viral reproduction; cytokine and chemokine mediated signaling pathway; JAK-STAT cascade; defense response to virus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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