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STAT2 recombinant protein

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Catalog # MBS515185
Unit / Price
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  0.02 mg  /  $270 +1 FREE 8GB USB
  0.05 mg  /  $450 +1 FREE 8GB USB
SDS-PAGE
Product Name

STAT2, Recombinant Protein

Popular Item
Full Product Name

STAT2 recombinant protein

Product Synonym Names
STAT113; ISGF-3; MGC59816; P113
Product Synonym Gene Name
STAT113; ISGF-3; MGC59816; P113[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000012.11 (56735381..56754058, complement). Location: 12q13.3
OMIM
600556
3D Structure
ModBase 3D Structure for P52630
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human STAT2 was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Other Notes
Small volumes of STAT2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
STAT2 recombinant protein
Recombinant full-length human STAT2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: STAT2 (Signal transducer and activator of transcription) is a family member of STAT transcription factors that are implicated in programming gene expression in biological events as diverse as embryonic development, programmed cell death, organogenesis, innate immunity, adaptive immunity and cell growth regulation (1). JAKs can phosphorylate and activate STATs. Activation of the JAK/STAT pathway in response to cytokine action can lead to regulation of gene expression during proliferation and differentiation of the immune cells. STATs have been shown to be involved in inflammation, autoimmune and allergic diseases (2).
Applications Tested/Suitable for STAT2 recombinant protein
Kinase Assay, Western Blot (WB)

NCBI/Uniprot data below describe general gene information for STAT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
~138 kDa
NCBI Official Full Name
Homo sapiens signal transducer and activator of transcription 2, 113kDa (STAT2), transcript variant 1, mRNA
NCBI Official Synonym Full Names
signal transducer and activator of transcription 2, 113kDa
NCBI Official Symbol
STAT2  [Similar Products]
NCBI Official Synonym Symbols
P113; ISGF-3; STAT113
  [Similar Products]
NCBI Protein Information
signal transducer and activator of transcription 2; interferon alpha induced transcriptional activator
UniProt Protein Name
Signal transducer and activator of transcription 2
UniProt Synonym Protein Names
p113
UniProt Gene Name
STAT2  [Similar Products]
UniProt Entry Name
STAT2_HUMAN
NCBI Summary for STAT2
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Comments for STAT2
Function: Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Ref.10

Subunit structure: Interacts with ISGF3G/IRF-9 in the cytoplasm. Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation. Interacts with CRSP2 and CRSP6. Interacts with Simian virus 5 protein V and rabies virus phosphoprotein

By similarity. Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation. Interacts with human cytomegalovirus/HHV-5 protein UL123; this interaction promotes viral growth. Ref.10 Ref.11 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18

Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus upon activation by IFN-alpha/beta. Ref.13 Ref.20

Post-translational modification: Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response. Ref.7 Ref.8 Ref.11 Ref.20

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.

Sequence caution: The sequence AAB36226.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAB36227.1 differs from that shown. Reason: Erroneous gene model prediction.
Product References and Citations for STAT2 recombinant protein
1. Horvath, C.M.: STAT proteins and transcriptional responses to extracellular signals. Trends Biochem Sci. 2000 Oct;25(10):496-502.
2. Pfitzner, E. et al: The role of STATs in inflammation and inflammatory diseases. Curr Pharm Des. 2004;10(23):2839-50.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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