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STMN elisa kit :: Human Stathmin (STMN) ELISA Kit

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Catalog # MBS7247839
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
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  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Stathmin (STMN), ELISA Kit

Popular Item
Full Product Name

Human Stathmin (STMN) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
151442
3D Structure
ModBase 3D Structure for P16949
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of STMN. No significant cross-reactivity or interference between STMN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STMN and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of STMN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for STMNpurchase
MBS7247839 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Stathmin (STMN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing STMN. The ELISA analytical biochemical technique of the MBS7247839 kit is based on STMN antibody-STMN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect STMN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, STMN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
STMN elisa kit
Intended Uses: This STMN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STMN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: STMN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STMN antibody and an STMN-HRP conjugate. The assay sample and buffer are incubated together with STMN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STMN concentration since STMN from samples and STMN-HRP conjugate compete for the anti-STMN antibody binding site. Since the number of sites is limited, as more sites are occupied by STMN from the sample, fewer sites are left to bind STMN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STMN concentration in each sample is interpolated from this standard curve.
Product Categories/Family for STMN elisa kit

Typical Standard Curve/Testing Data of STMN elisa kit
STMN elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS7247839. Click to request current manual
NCBI/Uniprot data below describe general gene information for STMN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,824 Da
NCBI Official Full Name
stathmin
NCBI Official Synonym Full Names
stathmin 1
NCBI Official Symbol
STMN1  [Similar Products]
NCBI Official Synonym Symbols
Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215
  [Similar Products]
NCBI Protein Information
stathmin; prosolin; metablastin; oncoprotein 18; phosphoprotein 19; phosphoprotein p19; stathmin 1/oncoprotein 18; transmembrane protein C1orf215; leukemia-associated phosphoprotein p18
UniProt Protein Name
Stathmin
UniProt Synonym Protein Names
Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; Op18; Phosphoprotein p19; pp19; Prosolin; Protein Pr22; pp17
UniProt Gene Name
STMN1  [Similar Products]
UniProt Synonym Gene Names
C1orf215; LAP18; OP18; Op18; pp19  [Similar Products]
UniProt Entry Name
STMN1_HUMAN
NCBI Summary for STMN
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
UniProt Comments for STMN
STMN1: a microtubule-associated protein involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Phosphorylation reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, nonleukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: microtubule; neuron projection; membrane; cytoplasm; intracellular; cytosol

Molecular Function: tubulin binding; signal transducer activity

Biological Process: mitotic spindle organization and biogenesis; axonogenesis; response to virus; negative regulation of microtubule polymerization; microtubule depolymerization; brain development; positive regulation of cell motility; signal transduction; neurite development; regulation of cytoskeleton organization and biogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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