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STMN1 elisa kit :: Rat Stathmin 1 ELISA Kit

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Catalog # MBS733001
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Stathmin 1 (STMN1), ELISA Kit

Popular Item
Also Known As

Rat Stathmin 1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
151442
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/ml
Intended Uses
This STMN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STMN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications
!Intended Uses
This STMN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STMN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of STMN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSTMN1purchase
MBS733001 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Stathmin 1 (STMN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing STMN1. The ELISA analytical biochemical technique of the MBS733001 kit is based on STMN1 antibody-STMN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect STMN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, STMN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
STMN1 elisa kit
Principle of the Assay: STMN1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for STMN1. Standards or samples are then added to the microtiter plate wells and STMN1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of STMN1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for STMN1 are added to each well to "sandwich" the STMN1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain STMN1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STMN1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for STMN1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of STMN1 elisa kit
STMN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS733001. Click to request current manual
NCBI/Uniprot data below describe general gene information for STMN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,824 Da
NCBI Official Full Name
stathmin 1, partial
NCBI Official Synonym Full Names
stathmin 1
NCBI Official Symbol
STMN1  [Similar Products]
NCBI Official Synonym Symbols
Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215
  [Similar Products]
NCBI Protein Information
stathmin; prosolin; metablastin; oncoprotein 18; phosphoprotein 19; phosphoprotein p19; stathmin 1/oncoprotein 18; transmembrane protein C1orf215; leukemia-associated phosphoprotein p18
UniProt Protein Name
Stathmin
UniProt Synonym Protein Names
Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; Op18; Phosphoprotein p19; pp19; Prosolin; Protein Pr22; pp17
Protein Family
UniProt Gene Name
STMN1  [Similar Products]
UniProt Synonym Gene Names
C1orf215; LAP18; OP18; Op18; pp19  [Similar Products]
UniProt Entry Name
STMN1_HUMAN
NCBI Summary for STMN1
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
UniProt Comments for STMN1
STMN1: a microtubule-associated protein involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Phosphorylation reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, nonleukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: microtubule; neuron projection; membrane; cytoplasm; intracellular; cytosol

Molecular Function: tubulin binding; signal transducer activity

Biological Process: mitotic spindle organization and biogenesis; axonogenesis; negative regulation of microtubule polymerization; response to virus; microtubule depolymerization; positive regulation of cell motility; brain development; signal transduction; neurite development; regulation of cytoskeleton organization and biogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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