• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Syn1 elisa kit :: Rat Synapsin-1 (Syn1) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS706242
Unit / Price DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Typical Testing Data/Standard Curve (for reference only)
Product Name

Synapsin-1 (Syn1), ELISA Kit

Popular Item
Also Known As

Rat Synapsin-1, Syn1 ELISA Kit

Product Synonym Names
Rat Synapsin-1 (Syn1) ELISA kit; RP1-230G1.2; SYN1a; SYN1b; SYNI; brain protein 4.1; Synapsin-1 (Syn1)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS706242 Testing Data
Sequence Length
668
3D Structure
ModBase 3D Structure for P09951
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat synapsin-1. No significant cross-reactivity or interference between rat synapsin-1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell culture supernates
Assay Type
Sandwich
Detection Range
0. 156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of rat synapsin-1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Syn1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSyn1purchase
MBS706242 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Synapsin-1 (Syn1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Syn1. The ELISA analytical biochemical technique of the MBS706242 kit is based on Syn1 antibody-Syn1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Syn1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Syn1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Syn1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for synapsin-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any synapsin-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for synapsin-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of synapsin-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of Syn1 elisa kit
Syn1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS706242. Click to request current manual
NCBI/Uniprot data below describe general gene information for Syn1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
73,988 Da
NCBI Official Full Name
synapsin-1 isoform b
NCBI Official Synonym Full Names
synapsin I
NCBI Official Symbol
NCBI Protein Information
synapsin-1; synapsin 1
UniProt Protein Name
Synapsin-1
UniProt Synonym Protein Names
Synapsin I
Protein Family
UniProt Gene Name
UniProt Entry Name
SYN1_RAT
NCBI Summary for Syn1
may act as an anchoring protein by linking the vesicle phospholipid layer and the cytoskeleton in neuronal presynaptic terminals; may play a role in neurotransmitter release [RGD, Feb 2006]
UniProt Comments for Syn1
Function: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with Syn1 elisa kit
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000