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SYP elisa kit :: Human synaptophysin ELISA Kit

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Catalog # MBS942026
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
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  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

synaptophysin (SYP), ELISA Kit

Popular Item
Also Known As

Human synaptophysin, SYP ELISA Kit

Product Synonym Names
Human synaptophysin (SYP) ELISA kit; MRXSYP; major synaptic vesicle protein P38; synaptophysin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS942026 Testing Data
Sequence Length
313
OMIM
313475
3D Structure
ModBase 3D Structure for P08247
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human SYP. No significant cross-reactivity or interference between human SYP and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
62.5 pg/ml -4000 pg/ml.
Sensitivity
The minimum detectable dose of human SYP is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SYP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSYPpurchase
MBS942026 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the synaptophysin (SYP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SYP. The ELISA analytical biochemical technique of the MBS942026 kit is based on SYP antibody-SYP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SYP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SYP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SYP elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SYP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SYP elisa kit
SYP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS942026. Click to request current manual
NCBI/Uniprot data below describe general gene information for SYP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,845 Da
NCBI Official Full Name
synaptophysin
NCBI Official Synonym Full Names
synaptophysin
NCBI Official Symbol
NCBI Official Synonym Symbols
MRX96; MRXSYP
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NCBI Protein Information
synaptophysin; major synaptic vesicle protein P38
UniProt Protein Name
Synaptophysin
UniProt Synonym Protein Names
Major synaptic vesicle protein p38
Protein Family
UniProt Gene Name
UniProt Entry Name
SYPH_HUMAN
NCBI Summary for SYP
This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]
UniProt Comments for SYP
SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, integral; Vesicle; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xp11.23-p11.22

Cellular Component: presynaptic membrane; synaptic vesicle; neuron projection; protein complex; synaptic vesicle membrane; presynaptic active zone; excitatory synapse; cell junction; integral to synaptic vesicle membrane

Molecular Function: identical protein binding; protein self-association; transporter activity; syntaxin-1 binding; cholesterol binding; protein complex binding; SH2 domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic vesicle maturation; synaptic vesicle membrane organization and biogenesis; endocytosis; regulation of short-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity

Disease: Mental Retardation, X-linked 96
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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