• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SYVN1 elisa kit :: Human synovial apoptosis inhibitor 1, synoviolin ELISA Kit

Scan QR to view Datasheet
Catalog # MBS939550
Unit / Price DISCONTINUED
You will be redirected to our replacement product
Typical Testing Data/Standard Curve (for reference only)
Product Name

synovial apoptosis inhibitor 1, synoviolin (SYVN1), ELISA Kit

Popular Item
Also Known As

Human E3 ubiquitin-protein ligase synoviolin, SYVN1 ELISA Kit

Product Synonym Names
Human E3 ubiquitin-protein ligase synoviolin (SYVN1) ELISA kit; HRD1; KIAA1810; MGC40372; synoviolin 1; synovial apoptosis inhibitor 1; synoviolin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS939550 Testing Data
Sequence Length
617
OMIM
608046
3D Structure
ModBase 3D Structure for Q86TM6
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human SYVN1. No significant cross-reactivity or interference between human SYVN1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
9.375 pg/ml -600 pg/ml.
Sensitivity
The minimum detectable dose of human SYVN1 is typically less than 2.34 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SYVN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSYVN1purchase
MBS939550 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the synovial apoptosis inhibitor 1, synoviolin (SYVN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SYVN1. The ELISA analytical biochemical technique of the MBS939550 kit is based on SYVN1 antibody-SYVN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SYVN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SYVN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SYVN1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SYVN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYVN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYVN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYVN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SYVN1 elisa kit
SYVN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS939550. Click to request current manual
NCBI/Uniprot data below describe general gene information for SYVN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67,685 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase synoviolin isoform a
NCBI Official Synonym Full Names
synovial apoptosis inhibitor 1, synoviolin
NCBI Official Symbol
SYVN1  [Similar Products]
NCBI Official Synonym Symbols
DER3; HRD1
  [Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog
UniProt Protein Name
E3 ubiquitin-protein ligase synoviolin
UniProt Synonym Protein Names
Synovial apoptosis inhibitor 1
UniProt Gene Name
SYVN1  [Similar Products]
UniProt Synonym Gene Names
HRD1; KIAA1810  [Similar Products]
UniProt Entry Name
SYVN1_HUMAN
NCBI Summary for SYVN1
This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011]
UniProt Comments for SYVN1
SYVN1: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Belongs to the HRD1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Membrane protein, integral; EC 6.3.2.19; Endoplasmic reticulum; Membrane protein, multi-pass; EC 6.3.2.-; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding

Biological Process: ER-associated protein catabolic process; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response; in utero embryonic development; protein ubiquitination; protein amino acid N-linked glycosylation via asparagine
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withSYVN1 elisa kit
 Reference Product  PubMed Publications
 TP53 elisa kit  >1 publications with SYVN1 and TP53
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000