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TAF4 elisa kit :: Mouse TAF4 ELISA Kit

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Catalog # MBS2510144
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

TAF4, ELISA Kit

Popular Item
Also Known As

Mouse TAF4 (TATA Box Binding Protein Associated Factor 4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
1085
OMIM
601796
3D Structure
ModBase 3D Structure for O00268
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse TAF4. No significant cross-reactivity or interference between Mouse TAF4 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TAF4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTAF4purchase
MBS2510144 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TAF4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TAF4. The ELISA analytical biochemical technique of the MBS2510144 kit is based on TAF4 antibody-TAF4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TAF4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TAF4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TAF4 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse TAF4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TAF4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TAF4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TAF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TAF4. You can calculate the concentration of TAF4 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of TAF4 elisa kit
TAF4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2510144. Click to request current manual
NCBI/Uniprot data below describe general gene information for TAF4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
110,114 Da
NCBI Official Full Name
transcription initiation factor TFIID subunit 4
NCBI Official Synonym Full Names
TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa
NCBI Official Symbol
NCBI Official Synonym Symbols
TAF2C; TAF4A; TAF2C1; TAFII130; TAFII135
  [Similar Products]
NCBI Protein Information
transcription initiation factor TFIID subunit 4; RNA polymerase II TBP-associated factor subunit C; TAF(II)130; TAF(II)135; TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135 kD; TAFII-130; TAFII-135; TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD; TBP-associated factor 4; transcription initiation factor TFIID 130 kDa subunit; transcription initiation factor TFIID 135 kD subunit; transcription initiation factor TFIID 135 kDa subunit
UniProt Protein Name
Transcription initiation factor TFIID subunit 4
UniProt Synonym Protein Names
RNA polymerase II TBP-associated factor subunit C; TBP-associated factor 4; Transcription initiation factor TFIID 130 kDa subunit; TAF(II)130; TAFII-130; TAFII130; Transcription initiation factor TFIID 135 kDa subunit; TAF(II)135; TAFII-135; TAFII135
UniProt Gene Name
UniProt Synonym Gene Names
TAF2C; TAF2C1; TAF4A; TAFII130; TAFII135; TAF(II)130; TAFII-130; TAFII130; TAF(II)135; TAFII-135; TAFII135  [Similar Products]
UniProt Entry Name
TAF4_HUMAN
NCBI Summary for TAF4
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. [provided by RefSeq, Jul 2008]
UniProt Comments for TAF4
TAF4: Makes part of TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone. Belongs to the TAF4 family.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nucleoplasm; transcription factor TFIID complex; cytoplasm

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; ovarian follicle development; positive regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; transcription initiation; gene expression
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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