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TAGLN2 elisa kit :: Human transgelin 2 ELISA Kit

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Catalog # MBS939523
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

transgelin 2 (TAGLN2), ELISA Kit

Popular Item
Also Known As

Human Transgelin-2, TAGLN2 ELISA Kit

Product Synonym Names
Human Transgelin-2 (TAGLN2) ELISA kit; RP11-48O20.1; HA1756; KIAA0120; OTTHUMP00000024409; SM22-alpha homolog; transgelin 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS939523 Testing Data
Sequence Length
199
OMIM
604634
3D Structure
ModBase 3D Structure for P37802
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human TAGLN2. No significant cross-reactivity or interference between human TAGLN2 and analogues was observed.
Samples
Serum, plasma, Cell lysates
Assay Type
Sandwich
Detection Range
0.312 ng/ml -20 ng/ml.
Sensitivity
The minimum detectable dose of human TAGLN2 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TAGLN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTAGLN2purchase
MBS939523 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transgelin 2 (TAGLN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TAGLN2. The ELISA analytical biochemical technique of the MBS939523 kit is based on TAGLN2 antibody-TAGLN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TAGLN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TAGLN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TAGLN2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TAGLN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAGLN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAGLN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAGLN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TAGLN2 elisa kit
TAGLN2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS939523. Click to request current manual
NCBI/Uniprot data below describe general gene information for TAGLN2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,391 Da
NCBI Official Full Name
transgelin-2 isoform b
NCBI Official Synonym Full Names
transgelin 2
NCBI Official Symbol
TAGLN2  [Similar Products]
NCBI Official Synonym Symbols
HA1756
  [Similar Products]
NCBI Protein Information
transgelin-2; SM22-alpha homolog; epididymis tissue protein Li 7e
UniProt Protein Name
Transgelin-2
UniProt Synonym Protein Names
Epididymis tissue protein Li 7e; SM22-alpha homolog
Protein Family
UniProt Gene Name
TAGLN2  [Similar Products]
UniProt Synonym Gene Names
KIAA0120; CDABP0035  [Similar Products]
UniProt Entry Name
TAGL2_HUMAN
NCBI Summary for TAGLN2
The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
UniProt Comments for TAGLN2
TAGLN2: a cytoskeletal protein of the calponin family. One of the earliest markers of differentiated smooth muscle. Expressed in the smooth, cardiac, and skeletal muscle cells during early embryogenesis. Becomes restricted to vascular and visceral smooth muscle cells in late fetal development and adulthood. Its expression is abolished by activated Ras. Ras-dependent and Ras-independent mechanisms can cause the down-regulation of transgelin in breast and colon cancer cells lines and tumor samples.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q21-q25

Cellular Component: vesicle

Molecular Function: actin filament binding; protein binding

Biological Process: epithelial cell differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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