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TAT-III elisa kit :: Bovine Thrombin-antithrombin III Complex, TAT-III ELISA Kit

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Catalog # MBS701364
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Thrombin-antithrombin III Complex, TAT-III, ELISA Kit

Popular Item
Also Known As

Bovine Thrombin-antithrombin III Complex, TAT-III ELISA Kit

Product Synonym Names
Bovine Thrombin-antithrombin III Complex; TAT-III ELISA Kit; Thrombin-antithrombin III Complex; TAT-III
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS701364 Testing Data
Sequence Length
100
3D Structure
ModBase 3D Structure for P05913
Species Reactivity
Sensitivity
1.56 pg/ml
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TAT-III elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTAT-IIIpurchase
MBS701364 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thrombin-antithrombin III Complex, TAT-III, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TAT-III. The ELISA analytical biochemical technique of the MBS701364 kit is based on TAT-III antibody-TAT-III antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TAT-III antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TAT-III. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TAT-III elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with TAT-III. Standards or samples are then added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for TAT-III, mix well and incubated. The more the amount of TAT-III in samples, the less HRP- conjugated antibody preparation specific for TAT-III bound by pre-coated TAT-III. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. And the color develops in opposite to the amount of TAT-III in the sample. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TAT-III elisa kit
TAT-III elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701364. Click to request current manual
NCBI/Uniprot data below describe general gene information for TAT-III. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
11,387 Da
NCBI Official Full Name
tat-III
UniProt Protein Name
Protein Tat
UniProt Synonym Protein Names
Transactivating regulatory protein
UniProt Gene Name
UniProt Entry Name
TAT_SIVVT
UniProt Comments for TAT-III
Function: Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA. The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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