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TCN2 elisa kit :: Rat transcobalamin II; macrocytic anemia ELISA Kit

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Catalog # MBS928945
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TCN2 elisa kit
Product Name

transcobalamin II; macrocytic anemia (TCN2), ELISA Kit

Also Known As

Rat Transcobalamin-2, TCN2 ELISA Kit

Product Synonym Names
Rat Transcobalamin-2 (TCN2) ELISA kit; D22S676; D22S750; TC2; transcobalamin II; transcobalamin II; macrocytic anemia
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
3D Structure
ModBase 3D Structure for Q9R0D6
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of Rat TCN2. No significant cross-reactivity or interference between Rat TCN2 and analogues was observed.
Assay Type
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
Protein Biological Process 1
Protein Biological Process 2
Anion transport
Protein Biological Process 3
Cobalt transport
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TCN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTCN2purchase
MBS928945 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transcobalamin II; macrocytic anemia (TCN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TCN2. The ELISA analytical biochemical technique of the MBS928945 kit is based on TCN2 antibody-TCN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TCN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TCN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TCN2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TCN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS928945. Click to request current manual
NCBI/Uniprot data below describe general gene information for TCN2. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
47,420 Da
NCBI Official Full Name
NCBI Official Synonym Full Names
transcobalamin 2
NCBI Official Symbol
NCBI Official Synonym Symbols
Tc2; Tcn2p
  [Similar Products]
NCBI Protein Information
transcobalamin-2; TC-2; TCII; TC II; transcobalamin II
UniProt Protein Name
UniProt Synonym Protein Names
Transcobalamin II; TC II; TCII
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TC-2; TC II; TCII  [Similar Products]
UniProt Entry Name
NCBI Summary for TCN2
component of plasma cobalamin (Cbl; vitamin B12) transport to all cells [RGD, Feb 2006]
UniProt Comments for TCN2
TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space

Molecular Function: metal ion binding; cobalamin binding

Biological Process: cobalamin metabolic process; cobalamin transport; cobalt ion transport
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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