• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TDG recombinant protein :: G/T mismatch-specific thymine DNA glycosylase (TDG) Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS1463344
Unit / Price
Scan QR to view Datasheet
  0.05 mg (E-Coli)  /  $825 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $905 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $1,105 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $1,140 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,215 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $1,230 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,380 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,390 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,645 +2 FREE 8GB USB
  1 mg (E-Coli)  /  $1,845 +2 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $2,140 +2 FREE 8GB USB
  1 mg (Yeast)  /  $2,210 +2 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $2,235 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $3,300 +4 FREE 8GB USB
TDG recombinant protein
Product Name

G/T mismatch-specific thymine DNA glycosylase (TDG), Recombinant Protein

Full Product Name

Recombinant Human G/T mismatch-specific thymine DNA glycosylase (TDG)

Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-410aa, full length protein
Sequence
MEAENAGSYS LQQAQAFYTF PFQQLMAEAP NMAVVNEQQM PEEVPAPAPA QEPVQEAPKG RKRKPRTTEP KQPVEPKKPV ESKKSGKSAK SKEKQEKITD TFKVKRKVDR FNGVSEAELL TKTLPDILTF NLDIVIIGIN PGLMAAYKGH HYPGPGNHFW KCLFMSGLSE VQLNHMDDHT LPGKYGIGFT NMVERTTPGS KDLSSKEFRE GGRILVQKLQ KYQPRIAVFN GKCIYEIFSK EVFGVKVKNL EFGLQPHKIP DTETLCYVMP SSSARCAQFP RAQDKVHYYI KLKDLRDQLK GIERNMDVQE VQYTFDLQLA QEDAKKMAVK EEKYDPGYEA AYGGAYGENP CSSEPCGFSS NGLIESVELR GESAFSGIPN GQWMTQSFTD QIPSFSNHCG TQEQEEESHA
OMIM
601423
3D Structure
ModBase 3D Structure for Q13569
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Human
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of TDG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TDG recombinant protein
This protein belongs to the TDG
mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G
T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C
T and T
T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
NCBI/Uniprot data below describe general gene information for TDG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,053 Da
NCBI Official Full Name
G/T mismatch-specific thymine DNA glycosylase
NCBI Official Synonym Full Names
thymine DNA glycosylase
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
G/T mismatch-specific thymine DNA glycosylase
UniProt Protein Name
G/T mismatch-specific thymine DNA glycosylase
UniProt Synonym Protein Names
Thymine-DNA glycosylase; hTDG
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for TDG
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]
UniProt Comments for TDG
DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000