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TF elisa kit :: Transferrin ELISA Kit

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Catalog # MBS564061
Unit / Price
  1x96 Wells  /  $405 +1 FREE 8GB USB
TF elisa kit
Product Name

Transferrin (TF), ELISA Kit

Popular Item
Also Known As

Human Transferrin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 3; NC_000003.11 (133464977..133497850). Location: 3q22.1
OMIM
190000
3D Structure
ModBase 3D Structure for P02787
Host
Human
Samples
Immunoperoxidase Assay for Determination of Transferrin in Human Samples
Assay Type
Sandwich
Intended Uses
The Transferrin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Transferrin in human biological samples.
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTFpurchase
MBS564061 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Human Transferrin (TF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TF. The ELISA analytical biochemical technique of the MBS564061 kit is based on TF antibody-TF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TF elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Transferrin present in samples reacts with the anti-Transferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-TX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound TX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo-genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of TX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of TX in the test sample. The quantity of TX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Transferrin (TX) is a metal-combining protein that reversibly binds to acid-soluble iron in plasma. It functions to transport iron to the bone marrow and to tissue storage organs such as the liver. Transferrin also participate in the regulation and control of iron absorption and protects against iron intoxication. Like haptoglobin, the carrier of hemoglobin, Transferrin is synthesized in the liver, but unlike haptoglobin transferrin is returned to the circulation after unloading its iron in the reticuloendothelial system. This ELISA kit can be used to measure transferrin in serum, tissue extracts and other biological fluids.
Product Categories/Family for TF elisa kit
Sample Manual Insert of MBS564061. Click to request current manual
NCBI/Uniprot data below describe general gene information for TF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
77,064 Da[Similar Products]
NCBI Official Full Name
transferrin
NCBI Official Synonym Full Names
transferrin
NCBI Official Symbol
NCBI Official Synonym Symbols
PRO1557; PRO2086; DKFZp781D0156
  [Similar Products]
NCBI Protein Information
serotransferrin; siderophilin; OTTHUMP00000197155; beta-1 metal-binding globulin
UniProt Protein Name
Serotransferrin
UniProt Synonym Protein Names
Beta-1 metal-binding globulin; Siderophilin
Protein Family
UniProt Gene Name
UniProt Entry Name
TRFE_HUMAN
NCBI Summary for TF
This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq]
UniProt Comments for TF
TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: extracellular space; cell surface; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; coated pit; recycling endosome; perinuclear region of cytoplasm; endocytic vesicle; late endosome; apical plasma membrane; basal plasma membrane; endosome membrane; vesicle

Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding

Biological Process: retinal homeostasis; platelet activation; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport

Disease: Atransferrinemia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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