• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TF recombinant protein :: Tissue Factor (TF) Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS2012021 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $145 +1 FREE 8GB USB
  0.05 mg  /  $280 +1 FREE 8GB USB
  0.1 mg  /  $440 +1 FREE 8GB USB
  0.2 mg  /  $540 +1 FREE 8GB USB
  0.5 mg  /  $1,040 +1 FREE 8GB USB
  1 mg  /  $1,545 +1 FREE 8GB USB
Product Name

Tissue Factor (TF), Recombinant Protein

Popular Item
Also Known As

Recombinant Tissue Factor (TF)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
295
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG S-G TPPGKAFNLT WISTDFKTIL EWQPKPTNYT YTVQISDRSR NWKYKCTGTT DTECDLTDEI VKDVNWTYEA RVLSVPWRNS THGKETLFGT HGEEPPFTNA RKFLPYRDTK IGQPVIQKYE QGGTKLKVTV KDSFTLVRKN GTFLTLRQVF GNDLGYILTY RKDSSTGRKT NTTHTNEFLI DVEKGVSYCF FAQAVIFSRK TNHKSPESIT KCTEQWKSVL GE
Chromosome Location
Chromosome: 2; NC_005101.3 (243348430..243360439). Location: 2q41
3D Structure
ModBase 3D Structure for P42533
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Gly30~Glu252 (Accession # P42533) with N-terminal His-Tag
Subcellular Location
Membrane; Single-pass type I membrane protein
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
9.2
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of TF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TF recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for TF recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Sequence Information of TF recombinant protein
TF recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for TF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
26.9kDa
NCBI Official Full Name
Tissue factor
NCBI Official Synonym Full Names
coagulation factor III (thromboplastin, tissue factor)
NCBI Official Symbol
NCBI Protein Information
tissue factor; TF; coagulation factor 3
UniProt Protein Name
Tissue factor
UniProt Synonym Protein Names
Coagulation factor III
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Cf3; TF  [Similar Products]
UniProt Entry Name
TF_RAT
NCBI Summary for TF
an initiation factor for blood coagulation; plays a critical role in hepatic ischemic reperfusion injury [RGD, Feb 2006]
UniProt Comments for TF
Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Subunit structure: Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Sequence similarities: Belongs to the tissue factor family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000