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TFF3 elisa kit :: Mouse Trefoil Factor 3, Intestinal (TFF3) ELISA Kit

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Catalog # MBS2024831 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $265 +1 FREE 8GB USB
  48-Strip-Wells  /  $490 +1 FREE 8GB USB
  96-Strip-Wells  /  $655 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,660 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,795 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Trefoil Factor 3, Intestinal (TFF3), ELISA Kit

Popular Item
Also Known As

Trefoil Factor 3, Intestinal (TFF3) ELISA Kit

Product Synonym Names
HITF; ITF3; TFI; HP1.B
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
94
OMIM
600633
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of TFF3. No significant cross-reactivity or interference between TFF3 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
1.56-100ng/mL
Sensitivity
0.65ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TFF3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TFF3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TFF3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTFF3purchase
MBS2024831 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Trefoil Factor 3, Intestinal (TFF3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TFF3. The ELISA analytical biochemical technique of the MBS2024831 kit is based on TFF3 antibody-TFF3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFF3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFF3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TFF3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TFF3 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TFF3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TFF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TFF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TFF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TFF3 elisa kit

Typical Testing Data/Standard Curve (for reference only) of TFF3 elisa kit
TFF3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2024831. Click to request current manual
NCBI/Uniprot data below describe general gene information for TFF3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
8,641 Da
NCBI Official Full Name
trefoil factor 3
NCBI Official Synonym Full Names
trefoil factor 3 (intestinal)
NCBI Official Symbol
NCBI Official Synonym Symbols
ITF; P1B; TFI
  [Similar Products]
NCBI Protein Information
trefoil factor 3
UniProt Protein Name
Trefoil factor 3
UniProt Synonym Protein Names
Intestinal trefoil factor; hITF; Polypeptide P1.B; hP1.B
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ITF; TFI; hITF; hP1.B  [Similar Products]
UniProt Entry Name
TFF3_HUMAN
NCBI Summary for TFF3
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
UniProt Comments for TFF3
TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule

Biological Process: response to peptide hormone stimulus; digestion; defense response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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