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TFR/CD71 elisa kit :: Human TFR/CD71 ELISA Kit

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Catalog # MBS2507578
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

TFR/CD71, ELISA Kit

Popular Item
Also Known As

Human TFR/CD71 (Transferrin Receptor) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS2507578 COA
Sequence Length
760
OMIM
190010
3D Structure
ModBase 3D Structure for P02786
Species Reactivity
Specificity
This kit recognizes natural and some recombinant Human TFR/CD71. No significant crossreactivity or interference between Human TFR/CD71 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TFR/CD71 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTFR/CD71purchase
MBS2507578 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TFR/CD71, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TFR/CD71. The ELISA analytical biochemical technique of the MBS2507578 kit is based on TFR/CD71 antibody-TFR/CD71 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFR/CD71 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFR/CD71. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TFR/CD71 elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human TFR/CD71 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human TFR/CD71. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human TFR/CD71 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human TFR/CD71, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TFR/CD71. The concentration of Human TFR/CD71 in samples can be calculated by comparing the OD of the samples with the standard curve.

Typical Testing Data/Standard Curve (for reference only) of TFR/CD71 elisa kit
TFR/CD71 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2507578. Click to request current manual
NCBI/Uniprot data below describe general gene information for TFR/CD71. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
84,871 Da
NCBI Official Full Name
transferrin receptor protein 1
NCBI Official Synonym Full Names
transferrin receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
T9; TR; TFR; p90; CD71; TFR1; TRFR
  [Similar Products]
NCBI Protein Information
transferrin receptor protein 1; transferrin receptor (p90, CD71)
UniProt Protein Name
Transferrin receptor protein 1
UniProt Synonym Protein Names
T9; p90; CD_antigen: CD71Transferrin receptor protein 1, serum form; sTfR
UniProt Gene Name
UniProt Synonym Gene Names
TR; TfR; TfR1; Trfr; sTfR  [Similar Products]
UniProt Entry Name
TFR1_HUMAN
NCBI Summary for TFR/CD71
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
UniProt Comments for TFR/CD71
TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; vesicle; endosome; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding

Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; regulation of cell growth; osteoclast differentiation; transmembrane transport; regulation of cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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