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TGF ALPHA recombinant protein

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Catalog # MBS232239
Unit / Price
  0.1 mg  /  $440 +1 FREE 8GB USB
TGF ALPHA recombinant protein
Product Name

TGF ALPHA, Recombinant Protein

Popular Item
Also Known As

RECOMBINANT HUMAN TGF ALPHA

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
159
OMIM
190170
3D Structure
ModBase 3D Structure for P01135
Species Reactivity
Mouse
Purity/Purification
>98% by SDS PAGE and HPLC
Form/Format
Recombinant protein - lyophilised
Concentration
Approximate Protein Concentration: 1.0 mg/ml after reconstitution (lot specific)
Reconstitution
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
Preparation
Activity
>1.4 x 106 Units/mg
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
Other Notes
Small volumes of TGF ALPHA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TGF ALPHA recombinant protein
Recombinant human TGFalpha is a 5.5kDa peptide corresponding to full-length mature human transforming growth factor alpha. TGFalpha is an EGF-related growth factor that stimulates the proliferation of epidermal and epithelial cells.
Applications Tested/Suitable for TGF ALPHA recombinant protein
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes for TGF ALPHA recombinant protein
ELISA: Recombinant human TGFalpha may be used as a standard for ELISA applications with either a purifed human TGFalpha antibody or a biotinylated human TGFalpha antibody.
Western Blot: Recombinant human TGFalpha may be used as the positive control for Western Blotting applications with either a purified human TGFalpha antibody or a biotinylated human TGFalpha antibody.
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well
Functional Assays: Minimum Dilution: 0.5; Maximum Dilution: 25ng/ml
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
NCBI/Uniprot data below describe general gene information for TGF ALPHA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Protein Molecular Weight: 5.5kD (50 Amino Acid Sequence)
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor, alpha
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
protransforming growth factor alpha; TGF-alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Synonym Protein Names
EGF-like TGF; ETGF; TGF type 1
UniProt Gene Name
UniProt Synonym Gene Names
TGF-alpha; ETGF  [Similar Products]
UniProt Entry Name
TGFA_HUMAN
NCBI Summary for TGF ALPHA
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for TGF ALPHA
TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: extracellular space; cell surface; basolateral plasma membrane; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding

Biological Process: response to drug; epidermal growth factor receptor signaling pathway; cell proliferation; wound healing; positive regulation of epidermal growth factor receptor activity; positive regulation of mitosis; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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