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TGF-beta elisa kit :: Hamster TGF-beta (Transforming Growth Factor beta) ELISA Kit

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Catalog # MBS7606539
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

TGF-beta (Transforming Growth Factor beta), ELISA Kit

Popular Item
Also Known As

Hamster TGF-beta (Transforming Growth Factor beta) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS7606539 COA
Sequence Length
112
OMIM
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of TGF-? . No significant cross-reactivity or interference between TGF-? and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Sandwich
Detection Range
31.25-2000pg/ml
Sensitivity
<18.75pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TGF- ? were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Nter-assay Precision (Precision between assays): 3 samples with low, middle and high level TGF-? were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TGF-beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTGF-betapurchase
MBS7606539 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TGF-beta (Transforming Growth Factor beta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGF-beta. The ELISA analytical biochemical technique of the MBS7606539 kit is based on TGF-beta antibody-TGF-beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGF-beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGF-beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TGF-beta elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TGF-? antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TGF-? antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGF-? amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGF-? can be calculated.

Typical Testing Data/Standard Curve (for reference only) of TGF-beta elisa kit
TGF-beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7606539. Click to request current manual
NCBI/Uniprot data below describe general gene information for TGF-beta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,341 Da
NCBI Official Full Name
transforming growth factor-beta, partial
NCBI Official Synonym Full Names
transforming growth factor beta 1
NCBI Official Symbol
TGFB1  [Similar Products]
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
  [Similar Products]
NCBI Protein Information
transforming growth factor beta-1
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1  [Similar Products]
UniProt Synonym Gene Names
TGFB; TGF-beta-1; LAP  [Similar Products]
NCBI Summary for TGF-beta
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]
UniProt Comments for TGF-beta
TGFB1: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3. May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with CD109, DPT and ASPN. Activated in vitro at pH below 3.5 and over 12.5. Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Belongs to the TGF-beta family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular region; extracellular space; Golgi lumen; microvillus; nucleus; plasma membrane; proteinaceous extracellular matrix

Molecular Function: antigen binding; cytokine activity; enzyme binding; glycoprotein binding; protein binding; punt binding; transforming growth factor beta receptor binding

Biological Process: active induction of host immune response by virus; ATP biosynthetic process; BMP signaling pathway; cell cycle arrest; cell development; cell migration; chondrocyte differentiation; common-partner SMAD protein phosphorylation; connective tissue replacement during inflammatory response; epidermal growth factor receptor signaling pathway; epithelial to mesenchymal transition; evasion of host defenses by virus; heart development; hemopoietic progenitor cell differentiation; hyaluronan catabolic process; inflammatory response; intercellular junction assembly and maintenance; leukocyte migration; lipopolysaccharide-mediated signaling pathway; lymph node development; MAPKKK cascade; membrane protein intracellular domain proteolysis; mitotic cell cycle checkpoint; negative regulation of blood vessel endothelial cell migration; negative regulation of cell cycle; negative regulation of cell differentiation; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cell-cell adhesion; negative regulation of DNA replication; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of mitotic cell cycle; negative regulation of myoblast differentiation; negative regulation of protein amino acid phosphorylation; negative regulation of skeletal muscle development; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; neural tube closure; neural tube development; phosphate metabolic process; platelet degranulation; positive regulation of blood vessel endothelial cell migration; positive regulation of bone mineralization; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cellular protein metabolic process; positive regulation of chemotaxis; positive regulation of collagen biosynthetic process; positive regulation of interleukin-17 production; positive regulation of isotype switching to IgA isotypes; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid dephosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein import into nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of protein secretion; positive regulation of superoxide release; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vascular permeability; protein amino acid phosphorylation; protein export from nucleus; protein import into nucleus, translocation; protein kinase B signaling cascade; receptor catabolic process; regulation of apoptosis; regulation of binding; regulation of cell migration; regulation of DNA binding; regulation of protein import into nucleus; regulation of striated muscle development; regulation of transforming growth factor beta receptor signaling pathway; response to estradiol stimulus; response to progesterone stimulus; response to wounding; salivary gland morphogenesis; SMAD protein complex assembly; SMAD protein nuclear translocation; transforming growth factor beta receptor signaling pathway; vasculogenesis; ventricular cardiac muscle morphogenesis

Disease: Camurati-engelmann Disease; Cystic Fibrosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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