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TGFA elisa kit :: Mouse Transforming Growth Factor Alpha ELISA Kit

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Catalog # MBS006972
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Transforming Growth Factor Alpha (TGFA), ELISA Kit

Popular Item
Full Product Name

Mouse Transforming Growth Factor Alpha ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Chromosome Location
Chromosome: 2; NC_000002.11 (70674412..70781147, complement). Location: 2p13
OMIM
190170
3D Structure
ModBase 3D Structure for P01135
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Assay Type
Sandwich
Detection Range
3.12pg/ml-100pg/ml.
Sensitivity
1.0pg/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TGFApurchase
MBS006972 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transforming Growth Factor Alpha (TGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGFA. The ELISA analytical biochemical technique of the MBS006972 kit is based on TGFA antibody-TGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TGFA elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of TGFA (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

Typical Testing Data/Standard Curve (for reference only) of TGFA elisa kit
TGFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS006972. Click to request current manual
NCBI/Uniprot data below describe general gene information for TGFA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,006 Da
NCBI Official Full Name
transforming growth factor-alpha
NCBI Official Synonym Full Names
transforming growth factor, alpha
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
protransforming growth factor alpha; TGF-alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Synonym Protein Names
EGF-like TGF; ETGF; TGF type 1
UniProt Gene Name
UniProt Synonym Gene Names
TGF-alpha; ETGF  [Similar Products]
UniProt Entry Name
TGFA_HUMAN
NCBI Summary for TGFA
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for TGFA
TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell cycle regulation; Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: extracellular space; cell surface; basolateral plasma membrane; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding

Biological Process: response to drug; epidermal growth factor receptor signaling pathway; cell proliferation; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; wound healing; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with TGFA elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with TGFA and Brain
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