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TGFA elisa kit :: Mouse transforming growth factor, alpha ELISA Kit

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Catalog # MBS704708
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

transforming growth factor, alpha (TGFA), ELISA Kit

Popular Item
Also Known As

Mouse transforming growth factor alpha, TGF-alpha ELISA Kit

Product Synonym Names
Mouse transforming growth factor alpha; TGF-alpha ELISA Kit; TFGA; transforming growth factor-alpha; transforming growth factor; alpha
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704708 Testing Data
Sequence Length
159
3D Structure
ModBase 3D Structure for P48030
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TGF-alpha. No significant cross-reactivity or interference between mouse TGF-alpha and analogues was observed.
Samples
Serum, plasma, cell culture supernates and tissue homogenates
Assay Type
Sandwich
Detection Range
12.5 pg/ml -800 pg/ml.
Sensitivity
The minimum detectable dose of mouse TGF-alpha is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTGFApurchase
MBS704708 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transforming growth factor, alpha (TGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGFA. The ELISA analytical biochemical technique of the MBS704708 kit is based on TGFA antibody-TGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TGFA elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of TGFA elisa kit
TGFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704708. Click to request current manual
NCBI/Uniprot data below describe general gene information for TGFA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
17,018 Da
NCBI Official Full Name
protransforming growth factor alpha preproprotein
NCBI Official Synonym Full Names
transforming growth factor alpha
NCBI Official Symbol
NCBI Official Synonym Symbols
wa1; wa-1
  [Similar Products]
NCBI Protein Information
protransforming growth factor alpha; waved 1; transforming growth factor-alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Synonym Protein Names
EGF-like TGF; ETGF; TGF type 1
UniProt Gene Name
UniProt Synonym Gene Names
TGF-alpha; ETGF  [Similar Products]
UniProt Entry Name
TGFA_MOUSE
UniProt Comments for TGFA
Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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