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TGFB1 elisa kit :: Pig transforming growth factor, beta 1 ELISA Kit

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Catalog # MBS701137
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

transforming growth factor, beta 1 (TGFB1), ELISA Kit

Popular Item
Full Product Name

Pig transforming growth factor beta1, TGF-beta1 ELISA Kit

Product Synonym Names
Pig transforming growth factor beta1 (TGF-beta1) ELISA Kit; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide; transforming growth factor; beta 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS701137 Testing Data
3D Structure
ModBase 3D Structure for P07200
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig TGFB1. No significant cross-reactivity or interference between Pig TGFB1 and analogues was observed.
Samples
Serum, plasma
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
0.195ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TGFB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTGFB1purchase
MBS701137 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transforming growth factor, beta 1 (TGFB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGFB1. The ELISA analytical biochemical technique of the MBS701137 kit is based on TGFB1 antibody-TGFB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TGFB1 elisa kit
Introduction: Transforming growth factor beta 1 or TGF-beta1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. TGF-beta1 was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids. TGF-beta1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-beta1. Some T cells (e.g. regulatory T cells) release TGF-beta1 to inhibit the actions of other T cells. Interleukin 1- and interleukin 2-dependent proliferation of activated T cells, and the activation of quiescent helper T cells and cytotoxic T cells is prevented by the activity of TGF-beta1. Similarly, TGF-beta1 can inhibit the secretion and activity of many other cytokines including interferon-gamma, tumor necrosis factor-alpha (TNF-alpha) and various interleukins. It can also decrease the expression levels of cytokine receptors, such as the IL-2 receptor to down-regulate the activity of immune cells. However, TGF-beta1 can also increase the expression of certain cytokines in T cells and promote their proliferation, particularly if the cells are immature. TGF-beta1 has similar effects on B cells that also vary according to the differentiation state of the cell. It inhibits proliferation and apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. The effects of TGF-beta1 on macrophages and monocytes are predominantly suppressive; this cytokine can inhibit the proliferation of these cells and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta1 can also have the opposite effect on cells of myeloid origin. For example, TGF-beta1 acts as a chemoattractant, directing an immune response to some pathogens; macrophages and monocytes respond to low levels of TGF-beta1 in a chemotactic manner. Furthermore, the expression of monocytic cytokines and phagocytic killing by macrophages can be increased by the action of TGF-beta1.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for TGF-beta1 and Horseradish Peroxidase (HRP) conjugated TGF-beta1, and incubated. A competitive inhibition reaction is launched between TGF-beta1 (Standards or samples) and Horseradish Peroxidase (HRP) conjugated TGF-beta1 with the antibody specific for TGF-beta1. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of TGFB1 elisa kit
TGFB1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701137. Click to request current manual
NCBI/Uniprot data below describe general gene information for TGFB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,294 Da
NCBI Official Full Name
transforming growth factor beta-1
NCBI Official Symbol
TGFB1  [Similar Products]
NCBI Official Synonym Symbols
TGF-BETA-1
  [Similar Products]
NCBI Protein Information
transforming growth factor beta-1; transforming growth factor-beta-1; transforming growth factor, beta 1 (Camurati-Engelmann disease)
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1  [Similar Products]
UniProt Synonym Gene Names
TGF-beta-1; LAP  [Similar Products]
UniProt Entry Name
TGFB1_PIG
UniProt Comments for TGFB1
Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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